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The condensin component NCAPG2 regulates microtubule–kinetochore attachment through recruitment of Polo-like kinase 1 to kinetochores

Author

Listed:
  • Jae Hyeong Kim

    (Research Institute, National Cancer Center)

  • Jaegal Shim

    (Research Institute, National Cancer Center)

  • Min-Ju Ji

    (Research Institute, National Cancer Center)

  • Yuna Jung

    (Research Institute, National Cancer Center)

  • Seoung Min Bong

    (Research Institute, National Cancer Center)

  • Young-Joo Jang

    (Laboratory of Cell Cycle and Signal Transduction, Dankook University)

  • Eun-Kyung Yoon

    (Research Institute, National Cancer Center)

  • Sang-Jin Lee

    (Research Institute, National Cancer Center)

  • Kwang Gi Kim

    (Research Institute, National Cancer Center)

  • Yon Hui Kim

    (Research Institute, National Cancer Center)

  • Changwoo Lee

    (Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine)

  • Byung Il Lee

    (Research Institute, National Cancer Center)

  • Kyung-Tae Kim

    (Research Institute, National Cancer Center)

Abstract

The early event of microtubule–kinetochore attachment is a critical stage for precise chromosome segregation. Here we report that NCAPG2, which is a component of the condensin II complex, mediates chromosome segregation through microtubule–kinetochore attachment by recruiting PLK1 to prometaphase kinetochores. NCAPG2 colocalizes with PLK1 at prometaphase kinetochores and directly interacts with the polo-box domain (PBD) of PLK1 via its highly conserved C-terminal region. In both humans and Caenorhabditis elegans, when NCAPG2 is depleted, the attachment of the spindle to the kinetochore is loosened and misoriented. This is caused by the disruption of PLK1 localization to the kinetochore and by the decreased phosphorylation of its kinetochore substrate, BubR1. In addition, the crystal structure of the PBD of PLK1, in complex with the C-terminal region of NCAPG2, 1007VLS-pT-L1011, exhibits structural conservation of PBD-phosphopeptides, suggesting that the regulation of NCAPG2 function is phosphorylation-dependent. These findings suggest that NCAPG2 plays an important role in regulating proper chromosome segregation through a functional interaction with PLK1 during mitosis.

Suggested Citation

  • Jae Hyeong Kim & Jaegal Shim & Min-Ju Ji & Yuna Jung & Seoung Min Bong & Young-Joo Jang & Eun-Kyung Yoon & Sang-Jin Lee & Kwang Gi Kim & Yon Hui Kim & Changwoo Lee & Byung Il Lee & Kyung-Tae Kim, 2014. "The condensin component NCAPG2 regulates microtubule–kinetochore attachment through recruitment of Polo-like kinase 1 to kinetochores," Nature Communications, Nature, vol. 5(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms5588
    DOI: 10.1038/ncomms5588
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