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Reconstituting ring-rafts in bud-mimicking topography of model membranes

Author

Listed:
  • Yong-Sang Ryu

    (School of Electrical Engineering #032, Seoul National University)

  • In-Ho Lee

    (School of Electrical Engineering #032, Seoul National University)

  • Jeng-Hun Suh

    (School of Electrical Engineering #032, Seoul National University)

  • Seung Chul Park

    (School of Electrical Engineering #032, Seoul National University)

  • Soojung Oh

    (World Class University (WCU) Program of Multiscale Mechanical Design, School of Mechanical and Aerospace Engineering, Seoul National University)

  • Luke R. Jordan

    (University of Minnesota)

  • Nathan J. Wittenberg

    (University of Minnesota)

  • Sang-Hyun Oh

    (University of Minnesota
    University of Minnesota)

  • Noo Li Jeon

    (World Class University (WCU) Program of Multiscale Mechanical Design, School of Mechanical and Aerospace Engineering, Seoul National University)

  • Byoungho Lee

    (School of Electrical Engineering #032, Seoul National University)

  • Atul N. Parikh

    (University of California
    Centre for Biomimetic Sensor Science, School of Materials Science and Engineering, Nanyang Technological University)

  • Sin-Doo Lee

    (School of Electrical Engineering #032, Seoul National University)

Abstract

During vesicular trafficking and release of enveloped viruses, the budding and fission processes dynamically remodel the donor cell membrane in a protein- or a lipid-mediated manner. In all cases, in addition to the generation or relief of the curvature stress, the buds recruit specific lipids and proteins from the donor membrane through restricted diffusion for the development of a ring-type raft domain of closed topology. Here, by reconstituting the bud topography in a model membrane, we demonstrate the preferential localization of cholesterol- and sphingomyelin-enriched microdomains in the collar band of the bud-neck interfaced with the donor membrane. The geometrical approach to the recapitulation of the dynamic membrane reorganization, resulting from the local radii of curvatures from nanometre-to-micrometre scales, offers important clues for understanding the active roles of the bud topography in the sorting and migration machinery of key signalling proteins involved in membrane budding.

Suggested Citation

  • Yong-Sang Ryu & In-Ho Lee & Jeng-Hun Suh & Seung Chul Park & Soojung Oh & Luke R. Jordan & Nathan J. Wittenberg & Sang-Hyun Oh & Noo Li Jeon & Byoungho Lee & Atul N. Parikh & Sin-Doo Lee, 2014. "Reconstituting ring-rafts in bud-mimicking topography of model membranes," Nature Communications, Nature, vol. 5(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms5507
    DOI: 10.1038/ncomms5507
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