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High-efficiency translational bypassing of non-coding nucleotides specified by mRNA structure and nascent peptide

Author

Listed:
  • Ekaterina Samatova

    (Max Planck Institute for Biophysical Chemistry)

  • Andrey L. Konevega

    (Max Planck Institute for Biophysical Chemistry
    B.P. Konstantinov Petersburg Nuclear Physics Institute, 188300 Gatchina, Russia, and St. Petersburg State Polytechnical University)

  • Norma M. Wills

    (University of Utah)

  • John F. Atkins

    (University of Utah
    School of Biochemistry and Cell Biology, University College Cork)

  • Marina V. Rodnina

    (Max Planck Institute for Biophysical Chemistry)

Abstract

The gene product 60 (gp60) of bacteriophage T4 is synthesized as a single polypeptide chain from a discontinuous reading frame as a result of bypassing of a non-coding mRNA region of 50 nucleotides by the ribosome. To identify the minimum set of signals required for bypassing, we recapitulated efficient translational bypassing in an in vitro reconstituted translation system from Escherichia coli. We find that the signals, which promote efficient and accurate bypassing, are specified by the gene 60 mRNA sequence. Systematic analysis of the mRNA suggests unexpected contributions of sequences upstream and downstream of the non-coding gap region as well as of the nascent peptide. During bypassing, ribosomes glide forward on the mRNA track in a processive way. Gliding may have a role not only for gp60 synthesis, but also during regular mRNA translation for reading frame selection during initiation or tRNA translocation during elongation.

Suggested Citation

  • Ekaterina Samatova & Andrey L. Konevega & Norma M. Wills & John F. Atkins & Marina V. Rodnina, 2014. "High-efficiency translational bypassing of non-coding nucleotides specified by mRNA structure and nascent peptide," Nature Communications, Nature, vol. 5(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms5459
    DOI: 10.1038/ncomms5459
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