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An infectious bat-derived chimeric influenza virus harbouring the entry machinery of an influenza A virus

Author

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  • Mindaugas Juozapaitis

    (Institute of Virology, Center for Microbiology and Hygiene, University Medical Center Freiburg, Hermann-Herder-Street 11, 79104 Freiburg, Germany)

  • Étori Aguiar Moreira

    (Institute of Virology, Center for Microbiology and Hygiene, University Medical Center Freiburg, Hermann-Herder-Street 11, 79104 Freiburg, Germany
    Spemann Graduate School of Biology and Medicine, University of Freiburg
    Faculty of Biology, University of Freiburg)

  • Ignacio Mena

    (Icahn School of Medicine at Mount Sinai
    Global Health and Emerging Pathogens Institute, Icahn School of Medicine at Mount Sinai)

  • Sebastian Giese

    (Institute of Virology, Center for Microbiology and Hygiene, University Medical Center Freiburg, Hermann-Herder-Street 11, 79104 Freiburg, Germany
    Faculty of Biology, University of Freiburg)

  • David Riegger

    (Institute of Virology, Center for Microbiology and Hygiene, University Medical Center Freiburg, Hermann-Herder-Street 11, 79104 Freiburg, Germany)

  • Anne Pohlmann

    (Institute of Diagnostic Virology, Friedrich-Loeffler-Institute)

  • Dirk Höper

    (Institute of Diagnostic Virology, Friedrich-Loeffler-Institute)

  • Gert Zimmer

    (Institute of Virology and Immunology (IVI))

  • Martin Beer

    (Institute of Diagnostic Virology, Friedrich-Loeffler-Institute)

  • Adolfo García-Sastre

    (Icahn School of Medicine at Mount Sinai
    Global Health and Emerging Pathogens Institute, Icahn School of Medicine at Mount Sinai
    Icahn School of Medicine at Mount Sinai)

  • Martin Schwemmle

    (Institute of Virology, Center for Microbiology and Hygiene, University Medical Center Freiburg, Hermann-Herder-Street 11, 79104 Freiburg, Germany)

Abstract

In 2012, the complete genomic sequence of a new and potentially harmful influenza A-like virus from bats (H17N10) was identified. However, infectious influenza virus was neither isolated from infected bats nor reconstituted, impeding further characterization of this virus. Here we show the generation of an infectious chimeric virus containing six out of the eight bat virus genes, with the remaining two genes encoding the haemagglutinin and neuraminidase proteins of a prototypic influenza A virus. This engineered virus replicates well in a broad range of mammalian cell cultures, human primary airway epithelial cells and mice, but poorly in avian cells and chicken embryos without further adaptation. Importantly, the bat chimeric virus is unable to reassort with other influenza A viruses. Although our data do not exclude the possibility of zoonotic transmission of bat influenza viruses into the human population, they indicate that multiple barriers exist that makes this an unlikely event.

Suggested Citation

  • Mindaugas Juozapaitis & Étori Aguiar Moreira & Ignacio Mena & Sebastian Giese & David Riegger & Anne Pohlmann & Dirk Höper & Gert Zimmer & Martin Beer & Adolfo García-Sastre & Martin Schwemmle, 2014. "An infectious bat-derived chimeric influenza virus harbouring the entry machinery of an influenza A virus," Nature Communications, Nature, vol. 5(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms5448
    DOI: 10.1038/ncomms5448
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