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Diverse and divergent protein post-translational modifications in two growth stages of a natural microbial community

Author

Listed:
  • Zhou Li

    (Oak Ridge National Laboratory
    Graduate School of Genome Science and Technology, University of Tennessee-Oak Ridge National Laboratory)

  • Yingfeng Wang

    (Oak Ridge National Laboratory)

  • Qiuming Yao

    (University of Missouri)

  • Nicholas B. Justice

    (University of California)

  • Tae-Hyuk Ahn

    (Oak Ridge National Laboratory)

  • Dong Xu

    (University of Missouri)

  • Robert L. Hettich

    (Oak Ridge National Laboratory
    Graduate School of Genome Science and Technology, University of Tennessee-Oak Ridge National Laboratory)

  • Jillian F. Banfield

    (University of California)

  • Chongle Pan

    (Oak Ridge National Laboratory
    Graduate School of Genome Science and Technology, University of Tennessee-Oak Ridge National Laboratory)

Abstract

Detailed characterization of post-translational modifications (PTMs) of proteins in microbial communities remains a significant challenge. Here we directly identify and quantify a broad range of PTMs (hydroxylation, methylation, citrullination, acetylation, phosphorylation, methylthiolation, S-nitrosylation and nitration) in a natural microbial community from an acid mine drainage site. Approximately 29% of the identified proteins of the dominant Leptospirillum group II bacteria are modified, and 43% of modified proteins carry multiple PTM types. Most PTM events, except S-nitrosylations, have low fractional occupancy. Notably, PTM events are detected on Cas proteins involved in antiviral defense, an aspect of Cas biochemistry not considered previously. Further, Cas PTM profiles from Leptospirillum group II differ in early versus mature biofilms. PTM patterns are divergent on orthologues of two closely related, but ecologically differentiated, Leptospirillum group II bacteria. Our results highlight the prevalence and dynamics of PTMs of proteins, with potential significance for ecological adaptation and microbial evolution.

Suggested Citation

  • Zhou Li & Yingfeng Wang & Qiuming Yao & Nicholas B. Justice & Tae-Hyuk Ahn & Dong Xu & Robert L. Hettich & Jillian F. Banfield & Chongle Pan, 2014. "Diverse and divergent protein post-translational modifications in two growth stages of a natural microbial community," Nature Communications, Nature, vol. 5(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms5405
    DOI: 10.1038/ncomms5405
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