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Virtual finger boosts three-dimensional imaging and microsurgery as well as terabyte volume image visualization and analysis

Author

Listed:
  • Hanchuan Peng

    (Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia 20147, USA
    Allen Institute for Brain Science, 551 North 34th Street, Suite 200, Seattle, Washington 98103, USA)

  • Jianyong Tang

    (Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia 20147, USA)

  • Hang Xiao

    (Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia 20147, USA)

  • Alessandro Bria

    (Integrated Research Centre, University Campus Bio-Medico of Rome
    University of Cassino and L.M.)

  • Jianlong Zhou

    (Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia 20147, USA)

  • Victoria Butler

    (Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia 20147, USA)

  • Zhi Zhou

    (Allen Institute for Brain Science, 551 North 34th Street, Suite 200, Seattle, Washington 98103, USA)

  • Paloma T. Gonzalez-Bellido

    (Development and Neuroscience, University of Cambridge
    Program in Sensory Physiology and Behavior, Marine Biological Laboratory)

  • Seung W. Oh

    (Allen Institute for Brain Science, 551 North 34th Street, Suite 200, Seattle, Washington 98103, USA)

  • Jichao Chen

    (M. D. Anderson Cancer Center
    Stanford University School of Medicine)

  • Ananya Mitra

    (Stanford University School of Medicine
    Circuit Therapeutics, Inc.)

  • Richard W. Tsien

    (Stanford University School of Medicine
    New York University Institute of Neuroscience, New York University)

  • Hongkui Zeng

    (Allen Institute for Brain Science, 551 North 34th Street, Suite 200, Seattle, Washington 98103, USA)

  • Giorgio A. Ascoli

    (Krasnow Institute for Advanced Study, George Mason University)

  • Giulio Iannello

    (Integrated Research Centre, University Campus Bio-Medico of Rome)

  • Michael Hawrylycz

    (Allen Institute for Brain Science, 551 North 34th Street, Suite 200, Seattle, Washington 98103, USA)

  • Eugene Myers

    (Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia 20147, USA
    Max Planck Institute of Molecular Cell Biology and Genetics)

  • Fuhui Long

    (Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia 20147, USA
    Allen Institute for Brain Science, 551 North 34th Street, Suite 200, Seattle, Washington 98103, USA
    BioImage, L.L.C.)

Abstract

Three-dimensional (3D) bioimaging, visualization and data analysis are in strong need of powerful 3D exploration techniques. We develop virtual finger (VF) to generate 3D curves, points and regions-of-interest in the 3D space of a volumetric image with a single finger operation, such as a computer mouse stroke, or click or zoom from the 2D-projection plane of an image as visualized with a computer. VF provides efficient methods for acquisition, visualization and analysis of 3D images for roundworm, fruitfly, dragonfly, mouse, rat and human. Specifically, VF enables instant 3D optical zoom-in imaging, 3D free-form optical microsurgery, and 3D visualization and annotation of terabytes of whole-brain image volumes. VF also leads to orders of magnitude better efficiency of automated 3D reconstruction of neurons and similar biostructures over our previous systems. We use VF to generate from images of 1,107 Drosophila GAL4 lines a projectome of a Drosophila brain.

Suggested Citation

  • Hanchuan Peng & Jianyong Tang & Hang Xiao & Alessandro Bria & Jianlong Zhou & Victoria Butler & Zhi Zhou & Paloma T. Gonzalez-Bellido & Seung W. Oh & Jichao Chen & Ananya Mitra & Richard W. Tsien & Ho, 2014. "Virtual finger boosts three-dimensional imaging and microsurgery as well as terabyte volume image visualization and analysis," Nature Communications, Nature, vol. 5(1), pages 1-13, September.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms5342
    DOI: 10.1038/ncomms5342
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