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Offspring production with sperm grown in vitro from cryopreserved testis tissues

Author

Listed:
  • Tetsuhiro Yokonishi

    (Yokohama City University Graduate School of Medicine)

  • Takuya Sato

    (Laboratory of Proteomics, Institute of Molecular Medicine and Life Science, Yokohama City University Association of Medical Science)

  • Mitsuru Komeya

    (Yokohama City University Graduate School of Medicine)

  • Kumiko Katagiri

    (Laboratory of Proteomics, Institute of Molecular Medicine and Life Science, Yokohama City University Association of Medical Science)

  • Yoshinobu Kubota

    (Yokohama City University Graduate School of Medicine)

  • Kazuhiko Nakabayashi

    (National Research Institute for Child Health and Development)

  • Kenichiro Hata

    (National Research Institute for Child Health and Development)

  • Kimiko Inoue

    (RIKEN, Bioresource Center)

  • Narumi Ogonuki

    (RIKEN, Bioresource Center)

  • Atsuo Ogura

    (RIKEN, Bioresource Center)

  • Takehiko Ogawa

    (Yokohama City University Graduate School of Medicine
    Laboratory of Proteomics, Institute of Molecular Medicine and Life Science, Yokohama City University Association of Medical Science)

Abstract

With the increasing cure rate of paediatric cancers, infertility, as one of the adverse effects of treatments, has become an important concern for patients and their families. Since semen cryopreservation is applicable only for post-pubertal patients, alternative pre-pubertal measures are necessary. Here we demonstrate that testis tissue cryopreservation is a realistic measure for preserving the fertility of an individual. Testis tissues of neonatal mice were cryopreserved either by slow freezing or by vitrification. After thawing, they were cultured on agarose gel and showed spermatogenesis up to sperm formation. Microinsemination was performed with round spermatids and sperm, leading to eight offspring in total. They grew healthily and produced progeny upon natural mating between them. This strategy, the cryopreservation of testis tissues followed by in vitro spermatogenesis, is promising to preserve the fertility of male paediatric cancer patients in the future.

Suggested Citation

  • Tetsuhiro Yokonishi & Takuya Sato & Mitsuru Komeya & Kumiko Katagiri & Yoshinobu Kubota & Kazuhiko Nakabayashi & Kenichiro Hata & Kimiko Inoue & Narumi Ogonuki & Atsuo Ogura & Takehiko Ogawa, 2014. "Offspring production with sperm grown in vitro from cryopreserved testis tissues," Nature Communications, Nature, vol. 5(1), pages 1-6, September.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms5320
    DOI: 10.1038/ncomms5320
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