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Specific recognition of the HIV-1 genomic RNA by the Gag precursor

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  • Ekram W. Abd El-Wahab

    (Architecture et Réactivité de l’ARN, Université de Strasbourg, CNRS, IBMC
    Present address: Tropical Health Department, High Institute of Public Health, Alexandria University, Egypt)

  • Redmond P. Smyth

    (Architecture et Réactivité de l’ARN, Université de Strasbourg, CNRS, IBMC)

  • Elodie Mailler

    (Architecture et Réactivité de l’ARN, Université de Strasbourg, CNRS, IBMC)

  • Serena Bernacchi

    (Architecture et Réactivité de l’ARN, Université de Strasbourg, CNRS, IBMC)

  • Valérie Vivet-Boudou

    (Architecture et Réactivité de l’ARN, Université de Strasbourg, CNRS, IBMC)

  • Marcel Hijnen

    (Centre for Virology, Burnet Institute
    Monash University)

  • Fabrice Jossinet

    (Architecture et Réactivité de l’ARN, Université de Strasbourg, CNRS, IBMC)

  • Johnson Mak

    (Centre for Virology, Burnet Institute
    Monash University
    Faculty of Health, School of Medicine, Deakin University
    Commonwealth Scientific and Industrial Research Organization, Livestock Industries, Australian Animal Health Laboratory)

  • Jean-Christophe Paillart

    (Architecture et Réactivité de l’ARN, Université de Strasbourg, CNRS, IBMC)

  • Roland Marquet

    (Architecture et Réactivité de l’ARN, Université de Strasbourg, CNRS, IBMC)

Abstract

During assembly of HIV-1 particles in infected cells, the viral Pr55Gag protein (or Gag precursor) must select the viral genomic RNA (gRNA) from a variety of cellular and viral spliced RNAs. However, there is no consensus on how Pr55Gag achieves this selection. Here, by using RNA binding and footprinting assays, we demonstrate that the primary Pr55Gag binding site on the gRNA consists of the internal loop and the lower part of stem-loop 1 (SL1), the upper part of which initiates gRNA dimerization. A double regulation ensures specific binding of Pr55Gag to the gRNA despite the fact that SL1 is also present in spliced viral RNAs. The region upstream of SL1, which is present in all HIV-1 RNAs, prevents binding to SL1, but this negative effect is counteracted by sequences downstream of SL4, which are unique to the gRNA.

Suggested Citation

  • Ekram W. Abd El-Wahab & Redmond P. Smyth & Elodie Mailler & Serena Bernacchi & Valérie Vivet-Boudou & Marcel Hijnen & Fabrice Jossinet & Johnson Mak & Jean-Christophe Paillart & Roland Marquet, 2014. "Specific recognition of the HIV-1 genomic RNA by the Gag precursor," Nature Communications, Nature, vol. 5(1), pages 1-13, September.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms5304
    DOI: 10.1038/ncomms5304
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