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Co-option of Sox3 as the male-determining factor on the Y chromosome in the fish Oryzias dancena

Author

Listed:
  • Yusuke Takehana

    (Laboratory of Bioresources, National Institute for Basic Biology
    the Graduate University for Advanced Studies (SOKENDAI))

  • Masaru Matsuda

    (Center for Bioscience Research and Education, Utsunomiya University, Utsunomiya)

  • Taijun Myosho

    (Institute of Science and Technology, Niigata University)

  • Maximiliano L. Suster

    (Neural Circuits and Behaviour Group, Uni Research AS
    National Institute of Genetics)

  • Koichi Kawakami

    (National Institute of Genetics
    the Graduate University for Advanced Studies (SOKENDAI))

  • Tadasu Shin-I

    (Center for Genetic Resource Information, National Institute of Genetics)

  • Yuji Kohara

    (Center for Genetic Resource Information, National Institute of Genetics)

  • Yoko Kuroki

    (Tohoku Medical Megabank Organization)

  • Atsushi Toyoda

    (the Graduate University for Advanced Studies (SOKENDAI)
    Comparative Genomics Laboratory, National Institute of Genetics)

  • Asao Fujiyama

    (the Graduate University for Advanced Studies (SOKENDAI)
    Comparative Genomics Laboratory, National Institute of Genetics
    National Institute of Informatics)

  • Satoshi Hamaguchi

    (Institute of Science and Technology, Niigata University)

  • Mitsuru Sakaizumi

    (Institute of Science and Technology, Niigata University)

  • Kiyoshi Naruse

    (Laboratory of Bioresources, National Institute for Basic Biology
    the Graduate University for Advanced Studies (SOKENDAI))

Abstract

Sex chromosomes harbour a primary sex-determining signal that triggers sexual development of the organism. However, diverse sex chromosome systems have been evolved in vertebrates. Here we use positional cloning to identify the sex-determining locus of a medaka-related fish, Oryzias dancena, and find that the locus on the Y chromosome contains a cis-regulatory element that upregulates neighbouring Sox3 expression in developing gonad. Sex-reversed phenotypes in Sox3Y transgenic fish, and Sox3Y loss-of-function mutants all point to its critical role in sex determination. Furthermore, we demonstrate that Sox3 initiates testicular differentiation by upregulating expression of downstream Gsdf, which is highly conserved in fish sex differentiation pathways. Our results not only provide strong evidence for the independent recruitment of Sox3 to male determination in distantly related vertebrates, but also provide direct evidence that a novel sex determination pathway has evolved through co-option of a transcriptional regulator potentially interacted with a conserved downstream component.

Suggested Citation

  • Yusuke Takehana & Masaru Matsuda & Taijun Myosho & Maximiliano L. Suster & Koichi Kawakami & Tadasu Shin-I & Yuji Kohara & Yoko Kuroki & Atsushi Toyoda & Asao Fujiyama & Satoshi Hamaguchi & Mitsuru Sa, 2014. "Co-option of Sox3 as the male-determining factor on the Y chromosome in the fish Oryzias dancena," Nature Communications, Nature, vol. 5(1), pages 1-10, September.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms5157
    DOI: 10.1038/ncomms5157
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