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ATG5 defines a phagophore domain connected to the endoplasmic reticulum during autophagosome formation in plants

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  • Romain Le Bars

    (Laboratoire Dynamique de la Compartimentation Cellulaire, CNRS UPR2355, Institut des Sciences du Végétal, Centre de Recherche de Gif (FRC3115), Saclay Plant Sciences)

  • Jessica Marion

    (Laboratoire Dynamique de la Compartimentation Cellulaire, CNRS UPR2355, Institut des Sciences du Végétal, Centre de Recherche de Gif (FRC3115), Saclay Plant Sciences)

  • Rémi Le Borgne

    (Laboratoire Dynamique de la Compartimentation Cellulaire, CNRS UPR2355, Institut des Sciences du Végétal, Centre de Recherche de Gif (FRC3115), Saclay Plant Sciences
    Pôle de Biologie Cellulaire, Imagif, Centre de Recherche de Gif, (FRC3115), Saclay Plant Sciences, CNRS)

  • Béatrice Satiat-Jeunemaitre

    (Laboratoire Dynamique de la Compartimentation Cellulaire, CNRS UPR2355, Institut des Sciences du Végétal, Centre de Recherche de Gif (FRC3115), Saclay Plant Sciences
    Pôle de Biologie Cellulaire, Imagif, Centre de Recherche de Gif, (FRC3115), Saclay Plant Sciences, CNRS)

  • Michele Wolfe Bianchi

    (Laboratoire Dynamique de la Compartimentation Cellulaire, CNRS UPR2355, Institut des Sciences du Végétal, Centre de Recherche de Gif (FRC3115), Saclay Plant Sciences
    UFR Sciences et Technologie, Université Paris-Est Créteil Val de Marne (UPEC), 61 av. Général de Gaulle, 94010 Créteil, France)

Abstract

Autophagosomes are the organelles responsible for macroautophagy and arise, in yeast and animals, from the sealing of a cup-shaped double-membrane precursor, the phagophore. How the phagophore is generated and grows into a sealed autophagosome is still not clear in detail, and unknown in plants. This is due, in part, to the scarcity of structurally informative, real-time imaging data of the required protein machinery at the phagophore formation site. Here we find that in intact living Arabidopsis tissue, autophagy-related protein ATG5, which is essential for autophagosome formation, is present at the phagophore site from early, sub-resolution stages and later defines a torus-shaped structure on a flat cisternal early phagophore. Movement and expansion of this structure are accompanied by the underlying endoplasmic reticulum, suggesting tight connections between the two compartments. Detailed real-time and 3D imaging of the growing phagophore are leveraged to propose a model for autophagosome formation in plants.

Suggested Citation

  • Romain Le Bars & Jessica Marion & Rémi Le Borgne & Béatrice Satiat-Jeunemaitre & Michele Wolfe Bianchi, 2014. "ATG5 defines a phagophore domain connected to the endoplasmic reticulum during autophagosome formation in plants," Nature Communications, Nature, vol. 5(1), pages 1-10, September.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms5121
    DOI: 10.1038/ncomms5121
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