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Plasticity and redundancy among AMA–RON pairs ensure host cell entry of Toxoplasma parasites

Author

Listed:
  • Mauld H. Lamarque

    (UMR 5235 CNRS, Université de Montpellier 2)

  • Magali Roques

    (UMR 5235 CNRS, Université de Montpellier 2)

  • Marie Kong-Hap

    (UMR 5235 CNRS, Université de Montpellier 2)

  • Michelle L. Tonkin

    (University of Victoria)

  • George Rugarabamu

    (CMU, University of Geneva)

  • Jean-Baptiste Marq

    (CMU, University of Geneva)

  • Diana M. Penarete-Vargas

    (UMR 5235 CNRS, Université de Montpellier 2)

  • Martin J. Boulanger

    (University of Victoria)

  • Dominique Soldati-Favre

    (CMU, University of Geneva)

  • Maryse Lebrun

    (UMR 5235 CNRS, Université de Montpellier 2)

Abstract

Malaria and toxoplasmosis are infectious diseases caused by the apicomplexan parasites Plasmodium and Toxoplasma gondii, respectively. These parasites have developed an invasion mechanism involving the formation of a moving junction (MJ) that anchors the parasite to the host cell and forms a ring through which the parasite penetrates. The composition and the assembly of the MJ, and in particular the presence of protein AMA1 and its interaction with protein RON2 at the MJ, have been the subject of intense controversy. Here, using reverse genetics, we show that AMA1, a vaccine candidate, interacts with RON2 to maintain the MJ structural integrity in T. gondii and is subsequently required for parasite internalization. Moreover, we show that disruption of the AMA1 gene results in upregulation of AMA1 and RON2 homologues that cooperate to support residual invasion. Our study highlights a considerable complexity and molecular plasticity in the architecture of the MJ.

Suggested Citation

  • Mauld H. Lamarque & Magali Roques & Marie Kong-Hap & Michelle L. Tonkin & George Rugarabamu & Jean-Baptiste Marq & Diana M. Penarete-Vargas & Martin J. Boulanger & Dominique Soldati-Favre & Maryse Leb, 2014. "Plasticity and redundancy among AMA–RON pairs ensure host cell entry of Toxoplasma parasites," Nature Communications, Nature, vol. 5(1), pages 1-13, September.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms5098
    DOI: 10.1038/ncomms5098
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    Cited by:

    1. Palak N. Patel & Thayne H. Dickey & Ababacar Diouf & Nichole D. Salinas & Holly McAleese & Tarik Ouahes & Carole A. Long & Kazutoyo Miura & Lynn E. Lambert & Niraj H. Tolia, 2023. "Structure-based design of a strain transcending AMA1-RON2L malaria vaccine," Nature Communications, Nature, vol. 14(1), pages 1-16, December.

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