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Dynamic haematopoietic cell contribution to the developing and adult epicardium

Author

Listed:
  • Gemma M. Balmer

    (Molecular Medicine Unit, UCL-Institute of Child Health)

  • Sveva Bollini

    (Regenerative Medicine Laboratory, University of Genoa & IRCCS AOU San Martino-IST, Largo Rosanna Benzi)

  • Karina N. Dubé

    (Molecular Medicine Unit, UCL-Institute of Child Health)

  • Juan Pedro Martinez-Barbera

    (Neural Development Unit, UCL-Institute of Child Health)

  • Owen Williams

    (Molecular Haematology and Cancer Biology Unit, UCL-Institute of Child Health)

  • Paul R. Riley

    (Anatomy and Genetics, University of Oxford)

Abstract

The epicardium is a cellular source with the potential to reconstitute lost cardiovascular tissue following myocardial infarction. Here we show that the adult epicardium contains a population of CD45+ haematopoietic cells (HCs), which are located proximal to coronary vessels and encased by extracellular matrix (ECM). This complex tertiary structure is established during the regenerative window between post-natal days 1 and 7. We show that these HCs proliferate within the first 24 h and are released between days 2 and 7 after myocardial infarction. The ECM subsequently reforms to encapsulate HCs after 21 days. Vav1-tdTomato labelling reveals an integral contribution of CD45+ HCs to the developing epicardium, which is not derived from the proepicardial organ. Transplantation experiments with either whole bone marrow or a Vav1+ subpopulation of cells confirm a contribution of HCs to the intact adult epicardium, which is elevated during the first 24 weeks of adult life but depleted in aged mice.

Suggested Citation

  • Gemma M. Balmer & Sveva Bollini & Karina N. Dubé & Juan Pedro Martinez-Barbera & Owen Williams & Paul R. Riley, 2014. "Dynamic haematopoietic cell contribution to the developing and adult epicardium," Nature Communications, Nature, vol. 5(1), pages 1-12, September.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms5054
    DOI: 10.1038/ncomms5054
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