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Optofluidic laser for dual-mode sensitive biomolecular detection with a large dynamic range

Author

Listed:
  • Xiang Wu

    (University of Michigan
    Key Laboratory for Micro and Nano Photonic Structures (Ministry of Education), Fudan University)

  • Maung Kyaw Khaing Oo

    (University of Michigan)

  • Karthik Reddy

    (University of Michigan
    University of Michigan)

  • Qiushu Chen

    (University of Michigan)

  • Yuze Sun

    (University of Texas at Arlington)

  • Xudong Fan

    (University of Michigan)

Abstract

Enzyme-linked immunosorbent assay (ELISA) is a powerful method for biomolecular analysis. The traditional ELISA employing light intensity as the sensing signal often encounters large background arising from non-specific bindings, material autofluorescence and leakage of excitation light, which deteriorates its detection limit and dynamic range. Here we develop the optofluidic laser-based ELISA, where ELISA occurs inside a laser cavity. The laser onset time is used as the sensing signal, which is inversely proportional to the enzyme concentration and hence the analyte concentration inside the cavity. We first elucidate the principle of the optofluidic laser-based ELISA, and then characterize the optofluidic laser performance. Finally, we present the dual-mode detection of interleukin-6 using commercial ELISA kits, where the sensing signals are simultaneously obtained by the traditional and the optofluidic laser-based ELISA, showing a detection limit of 1 fg ml−1 (38 aM) and a dynamic range of 6 orders of magnitude.

Suggested Citation

  • Xiang Wu & Maung Kyaw Khaing Oo & Karthik Reddy & Qiushu Chen & Yuze Sun & Xudong Fan, 2014. "Optofluidic laser for dual-mode sensitive biomolecular detection with a large dynamic range," Nature Communications, Nature, vol. 5(1), pages 1-7, September.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms4779
    DOI: 10.1038/ncomms4779
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