IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v5y2014i1d10.1038_ncomms4719.html
   My bibliography  Save this article

Klf4 and Klf5 differentially inhibit mesoderm and endoderm differentiation in embryonic stem cells

Author

Listed:
  • Irène Aksoy

    (Inserm, U846, 18 Avenue Doyen Lepine
    Stem Cell and Brain Research Institute
    Université de Lyon, Université Lyon 1
    Genome Institute of Singapore, 60 Biopolis street)

  • Vincent Giudice

    (Inserm, U846, 18 Avenue Doyen Lepine
    Stem Cell and Brain Research Institute
    Université de Lyon, Université Lyon 1)

  • Edwige Delahaye

    (Inserm, U846, 18 Avenue Doyen Lepine
    Stem Cell and Brain Research Institute
    Université de Lyon, Université Lyon 1)

  • Florence Wianny

    (Inserm, U846, 18 Avenue Doyen Lepine
    Stem Cell and Brain Research Institute
    Université de Lyon, Université Lyon 1)

  • Maxime Aubry

    (Inserm, U846, 18 Avenue Doyen Lepine
    Stem Cell and Brain Research Institute
    Université de Lyon, Université Lyon 1)

  • Magali Mure

    (Inserm, U846, 18 Avenue Doyen Lepine
    Stem Cell and Brain Research Institute
    Université de Lyon, Université Lyon 1)

  • Jiaxuan Chen

    (Genome Institute of Singapore, 60 Biopolis street)

  • Ralf Jauch

    (Genome Institute of Singapore, 60 Biopolis street
    Genome Regulation Laboratory, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences)

  • Gireesh K. Bogu

    (Genome Institute of Singapore, 60 Biopolis street)

  • Tobias Nolden

    (Max Planck Institute for Molecular Genetics, Ihnestrasse 63-73)

  • Heinz Himmelbauer

    (Max Planck Institute for Molecular Genetics, Ihnestrasse 63-73
    Center for Genomic Regulation (CRG), C. Dr. Aiguader 88
    Universitat Pompeu Fabra (UPF), C. Dr. Aiguader 88)

  • Michael Xavier Doss

    (Universitat Pompeu Fabra (UPF), C. Dr. Aiguader 88
    Present address: Stem Cell Center, Masonic Medical Research Laboratory, Utica, New York-13501, USA)

  • Agapios Sachinidis

    (Center of Physiology and Pathophysiology, Institute of Neurophysiology, Robert-Koch-Strasse. 39)

  • Herbert Schulz

    (Max Delbrück Center for Molecular Medicine, Robert-Rössle-Strasse 10)

  • Oliver Hummel

    (Max Delbrück Center for Molecular Medicine, Robert-Rössle-Strasse 10)

  • Paola Martinelli

    (Centro Nacional de Investigaciones Oncológicas, Melchor Fernández Almagro 3)

  • Norbert Hübner

    (Max Delbrück Center for Molecular Medicine, Robert-Rössle-Strasse 10)

  • Lawrence W. Stanton

    (Genome Institute of Singapore, 60 Biopolis street)

  • Francisco X. Real

    (Centro Nacional de Investigaciones Oncológicas, Melchor Fernández Almagro 3
    Departament de Ciències Experimentals i de la Salut, Universitat Pompeu Fabra)

  • Pierre-Yves Bourillot

    (Inserm, U846, 18 Avenue Doyen Lepine
    Stem Cell and Brain Research Institute
    Université de Lyon, Université Lyon 1)

  • Pierre Savatier

    (Inserm, U846, 18 Avenue Doyen Lepine
    Stem Cell and Brain Research Institute
    Université de Lyon, Université Lyon 1)

Abstract

Krüppel-like factors (Klf) 4 and 5 are two closely related members of the Klf family, known to play key roles in cell cycle regulation, somatic cell reprogramming and pluripotency. Here we focus on the functional divergence between Klf4 and Klf5 in the inhibition of mouse embryonic stem (ES) cell differentiation. Using microarrays and chromatin immunoprecipitation coupled to ultra-high-throughput DNA sequencing, we show that Klf4 negatively regulates the expression of endodermal markers in the undifferentiated ES cells, including transcription factors involved in the commitment of pluripotent stem cells to endoderm differentiation. Knockdown of Klf4 enhances differentiation towards visceral and definitive endoderm. In contrast, Klf5 negatively regulates the expression of mesodermal markers, some of which control commitment to the mesoderm lineage, and knockdown of Klf5 specifically enhances differentiation towards mesoderm. We conclude that Klf4 and Klf5 differentially inhibit mesoderm and endoderm differentiation in murine ES cells.

Suggested Citation

  • Irène Aksoy & Vincent Giudice & Edwige Delahaye & Florence Wianny & Maxime Aubry & Magali Mure & Jiaxuan Chen & Ralf Jauch & Gireesh K. Bogu & Tobias Nolden & Heinz Himmelbauer & Michael Xavier Doss &, 2014. "Klf4 and Klf5 differentially inhibit mesoderm and endoderm differentiation in embryonic stem cells," Nature Communications, Nature, vol. 5(1), pages 1-15, September.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms4719
    DOI: 10.1038/ncomms4719
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms4719
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms4719?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. K. A. Gajewska & H. Lescesen & M. Ramialison & K. M. Wagstaff & D. A. Jans, 2021. "Nuclear transporter Importin-13 plays a key role in the oxidative stress transcriptional response," Nature Communications, Nature, vol. 12(1), pages 1-13, December.
    2. Hans-Juergen Schulten & Deema Hussein, 2019. "Array expression meta-analysis of cancer stem cell genes identifies upregulation of PODXL especially in DCC low expression meningiomas," PLOS ONE, Public Library of Science, vol. 14(5), pages 1-20, May.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms4719. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.