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Generation of colonic IgA-secreting cells in the caecal patch

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  • Kazunori Masahata

    (Laboratory of Immune Regulation, Graduate School of Medicine, WPI Immunology Frontier Research Center, Osaka University, Suita
    Graduate School of Medicine, Osaka University, Suita)

  • Eiji Umemoto

    (Laboratory of Immune Regulation, Graduate School of Medicine, WPI Immunology Frontier Research Center, Osaka University, Suita
    Core Research for Evolutional Science and Technology, Japan Science and Technology Agency)

  • Hisako Kayama

    (Laboratory of Immune Regulation, Graduate School of Medicine, WPI Immunology Frontier Research Center, Osaka University, Suita
    Core Research for Evolutional Science and Technology, Japan Science and Technology Agency)

  • Manato Kotani

    (Graduate School of Medicine, WPI Immunology Frontier Research Center, Osaka University, Suita)

  • Shota Nakamura

    (Genome Information Research Center, Research Institute for Microbial Diseases, Osaka University, Suita)

  • Takashi Kurakawa

    (Laboratory of Immune Regulation, Graduate School of Medicine, WPI Immunology Frontier Research Center, Osaka University, Suita)

  • Junichi Kikuta

    (Graduate School of Medicine, WPI Immunology Frontier Research Center, Osaka University, Suita)

  • Kazuyoshi Gotoh

    (Genome Information Research Center, Research Institute for Microbial Diseases, Osaka University, Suita)

  • Daisuke Motooka

    (Genome Information Research Center, Research Institute for Microbial Diseases, Osaka University, Suita)

  • Shintaro Sato

    (Institute of Medical Science, The University of Tokyo)

  • Tomonori Higuchi

    (Kinki University Faculty of Medicine, Osaka-Sayama)

  • Yoshihiro Baba

    (Laboratory of Lymphocyte Differentiation, WPI Immunology Frontier Research Center, Osaka University, Suita)

  • Tomohiro Kurosaki

    (Laboratory of Lymphocyte Differentiation, WPI Immunology Frontier Research Center, Osaka University, Suita)

  • Makoto Kinoshita

    (Laboratory of Immune Regulation, Graduate School of Medicine, WPI Immunology Frontier Research Center, Osaka University, Suita
    Core Research for Evolutional Science and Technology, Japan Science and Technology Agency)

  • Yosuke Shimada

    (Laboratory of Immune Regulation, Graduate School of Medicine, WPI Immunology Frontier Research Center, Osaka University, Suita)

  • Taishi Kimura

    (Laboratory of Immune Regulation, Graduate School of Medicine, WPI Immunology Frontier Research Center, Osaka University, Suita)

  • Ryu Okumura

    (Laboratory of Immune Regulation, Graduate School of Medicine, WPI Immunology Frontier Research Center, Osaka University, Suita)

  • Akira Takeda

    (Laboratory of Immune Regulation, Graduate School of Medicine, WPI Immunology Frontier Research Center, Osaka University, Suita)

  • Masaru Tajima

    (The Institute of Experimental Animal Sciences, Faculty of Medicine, Osaka University, Suita)

  • Osamu Yoshie

    (Kinki University Faculty of Medicine, Osaka-Sayama)

  • Masahiro Fukuzawa

    (Graduate School of Medicine, Osaka University, Suita)

  • Hiroshi Kiyono

    (Core Research for Evolutional Science and Technology, Japan Science and Technology Agency
    Institute of Medical Science, The University of Tokyo)

  • Sidonia Fagarasan

    (Laboratory for Mucosal Immunity, Center for Integrative Medical Sciences, RIKEN)

  • Tetsuya Iida

    (Genome Information Research Center, Research Institute for Microbial Diseases, Osaka University, Suita
    Laboratory of Genomic Research on Pathogenic Bacteria, International Research Center for Infectious Diseases, Research Institute for Microbial Diseases, Osaka University, Suita)

  • Masaru Ishii

    (Core Research for Evolutional Science and Technology, Japan Science and Technology Agency
    Graduate School of Medicine, WPI Immunology Frontier Research Center, Osaka University, Suita)

  • Kiyoshi Takeda

    (Laboratory of Immune Regulation, Graduate School of Medicine, WPI Immunology Frontier Research Center, Osaka University, Suita
    Core Research for Evolutional Science and Technology, Japan Science and Technology Agency)

Abstract

Gut-associated lymphoid tissues are responsible for the generation of IgA-secreting cells. However, the function of the caecal patch, a lymphoid tissue in the appendix, remains unknown. Here we analyse the role of the caecal patch using germ-free mice colonized with intestinal bacteria after appendectomy. Appendectomized mice show delayed accumulation of IgA+ cells in the large intestine, but not the small intestine, after colonization. Decreased colonic IgA+ cells correlate with altered faecal microbiota composition. Experiments using photoconvertible Kaede-expressing mice or adoptive transfer show that the caecal patch IgA+ cells migrate to the large and small intestines, whereas Peyer’s patch cells are preferentially recruited to the small intestine. IgA+ cells in the caecal patch express higher levels of CCR10. Dendritic cells in the caecal patch, but not Peyer’s patches, induce CCR10 on cocultured B cells. Thus, the caecal patch is a major site for generation of IgA-secreting cells that migrate to the large intestine.

Suggested Citation

  • Kazunori Masahata & Eiji Umemoto & Hisako Kayama & Manato Kotani & Shota Nakamura & Takashi Kurakawa & Junichi Kikuta & Kazuyoshi Gotoh & Daisuke Motooka & Shintaro Sato & Tomonori Higuchi & Yoshihiro, 2014. "Generation of colonic IgA-secreting cells in the caecal patch," Nature Communications, Nature, vol. 5(1), pages 1-13, September.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms4704
    DOI: 10.1038/ncomms4704
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