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A red-shifted fluorescent substrate for aldehyde dehydrogenase

Author

Listed:
  • Il Minn

    (Russel H. Morgan Department of Radiology and Radiological Science)

  • Haofan Wang

    (Russel H. Morgan Department of Radiology and Radiological Science)

  • Ronnie C. Mease

    (Russel H. Morgan Department of Radiology and Radiological Science)

  • Youngjoo Byun

    (College of Pharmacy, Korea University, 2511 Sejong-ro, Jochiwon-eup, Sejong 339-700, South Korea)

  • Xing Yang

    (Russel H. Morgan Department of Radiology and Radiological Science)

  • Julia Wang

    (Department of Surgery and the McKusick-Nathans Institute of Genetic Medicine)

  • Steven D. Leach

    (Department of Surgery and the McKusick-Nathans Institute of Genetic Medicine)

  • Martin G. Pomper

    (Russel H. Morgan Department of Radiology and Radiological Science
    Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University)

Abstract

Selection of cells positive for aldehyde dehydrogenase (ALDH) activity from a green-fluorescent background is difficult with existing reagents. Here we report a red-shifted fluorescent substrate for ALDH, AldeRed 588-A, for labelling viable ALDHpos cells. We demonstrate that AldeRed 588-A successfully isolates ALDHhi human haematopoietic stem cells from heterogeneous cord blood mononuclear cells. AldeRed 588-A can be used for multicolour applications to fractionate ALDHpos cells in the presence of green fluorophores including the ALDEFLUOR reagent and cells expressing enhanced green-fluorescent protein (eGFP). AldeRed 588-A stains ALDHpos murine pancreatic centroacinar and terminal duct cells, as visualized using fluorescent microscopy. AldeRed588-A provides a useful tool to select stem cells or study ALDH within a green-fluorescent background.

Suggested Citation

  • Il Minn & Haofan Wang & Ronnie C. Mease & Youngjoo Byun & Xing Yang & Julia Wang & Steven D. Leach & Martin G. Pomper, 2014. "A red-shifted fluorescent substrate for aldehyde dehydrogenase," Nature Communications, Nature, vol. 5(1), pages 1-9, May.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms4662
    DOI: 10.1038/ncomms4662
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