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Structural basis for oligomerization of auxin transcriptional regulators

Author

Listed:
  • Max H. Nanao

    (European Molecular Biology Laboratory
    Unit of Virus Host-Cell Interactions, UJF-EMBL-CNRS, UMI 3265, 6 rue Jules Horowitz)

  • Thomas Vinos-Poyo

    (CNRS, Laboratoire de Physiologie Cellulaire and Végétale, UMR 5168
    Univ. Grenoble Alpes, LPCV
    CEA, DSV, iRTSV, LPCV
    INRA, LPCV)

  • Géraldine Brunoud

    (Laboratoire de Reproduction et Développement des Plantes, CNRS, INRA, ENS Lyon, UCBL, Université de Lyon)

  • Emmanuel Thévenon

    (CNRS, Laboratoire de Physiologie Cellulaire and Végétale, UMR 5168
    Univ. Grenoble Alpes, LPCV
    CEA, DSV, iRTSV, LPCV
    INRA, LPCV)

  • Meryl Mazzoleni

    (CNRS, Laboratoire de Physiologie Cellulaire and Végétale, UMR 5168
    Univ. Grenoble Alpes, LPCV
    CEA, DSV, iRTSV, LPCV
    INRA, LPCV)

  • David Mast

    (Laboratoire de Reproduction et Développement des Plantes, CNRS, INRA, ENS Lyon, UCBL, Université de Lyon)

  • Stéphanie Lainé

    (Laboratoire de Reproduction et Développement des Plantes, CNRS, INRA, ENS Lyon, UCBL, Université de Lyon)

  • Shucai Wang

    (Key Laboratory of Molecular Epigenetics of MOE, Institute of Genetics and Cytology, Institute of Grassland Science, Northeast Normal University)

  • Gretchen Hagen

    (University of Missouri)

  • Hanbing Li

    (University of Missouri)

  • Thomas J. Guilfoyle

    (University of Missouri)

  • François Parcy

    (CNRS, Laboratoire de Physiologie Cellulaire and Végétale, UMR 5168
    Univ. Grenoble Alpes, LPCV
    CEA, DSV, iRTSV, LPCV
    INRA, LPCV)

  • Teva Vernoux

    (Laboratoire de Reproduction et Développement des Plantes, CNRS, INRA, ENS Lyon, UCBL, Université de Lyon)

  • Renaud Dumas

    (CNRS, Laboratoire de Physiologie Cellulaire and Végétale, UMR 5168
    Univ. Grenoble Alpes, LPCV
    CEA, DSV, iRTSV, LPCV
    INRA, LPCV)

Abstract

The plant hormone auxin is a key morphogenetic regulator acting from embryogenesis onwards. Transcriptional events in response to auxin are mediated by the auxin response factor (ARF) transcription factors and the Aux/IAA (IAA) transcriptional repressors. At low auxin concentrations, IAA repressors associate with ARF proteins and recruit corepressors that prevent auxin-induced gene expression. At higher auxin concentrations, IAAs are degraded and ARFs become free to regulate auxin-responsive genes. The interaction between ARFs and IAAs is thus central to auxin signalling and occurs through the highly conserved domain III/IV present in both types of proteins. Here, we report the crystal structure of ARF5 domain III/IV and reveal the molecular determinants of ARF–IAA interactions. We further provide evidence that ARFs have the potential to oligomerize, a property that could be important for gene regulation in response to auxin.

Suggested Citation

  • Max H. Nanao & Thomas Vinos-Poyo & Géraldine Brunoud & Emmanuel Thévenon & Meryl Mazzoleni & David Mast & Stéphanie Lainé & Shucai Wang & Gretchen Hagen & Hanbing Li & Thomas J. Guilfoyle & François P, 2014. "Structural basis for oligomerization of auxin transcriptional regulators," Nature Communications, Nature, vol. 5(1), pages 1-8, May.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms4617
    DOI: 10.1038/ncomms4617
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