IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v5y2014i1d10.1038_ncomms4597.html
   My bibliography  Save this article

The 19S proteasome subunit Rpt3 regulates distribution of CENP-A by associating with centromeric chromatin

Author

Listed:
  • Teppei Kitagawa

    (Graduate School of Biostudies, Kyoto University, Yoshida-Konoe cho, Sakyo ku, Kyoto 606-8501, Japan)

  • Kojiro Ishii

    (Graduate School of Frontier Biosciences, Osaka University
    Institute for Academic Initiatives, Osaka University)

  • Kojiro Takeda

    (Institute for Integrative Neurobiology, Graduate School of Natural Science, Konan University, Kobe
    Faculty of Science and Engineering, Konan University, Kobe)

  • Tomohiro Matsumoto

    (Graduate School of Biostudies, Kyoto University, Yoshida-Konoe cho, Sakyo ku, Kyoto 606-8501, Japan
    Radiation Biology Center, Kyoto University, Yoshida-Konoe cho, Sakyo ku, Kyoto 606-8501, Japan)

Abstract

CENP-A, a variant of histone H3, is incorporated into centromeric chromatin and plays a role during kinetochore establishment. In fission yeast, the localization of CENP-A is limited to a region spanning 10–20 kb of the core domain of the centromere. Here, we report a mutant (rpt3-1) in which this region is expanded to 40–70 kb. Likely due to abnormal distribution of CENP-A, this mutant exhibits chromosome instability and enhanced gene silencing. Interestingly, the rpt3+ gene encodes a subunit of the 19S proteasome, which localizes to the nuclear membrane. Although Rpt3 associates with centromeric chromatin, the mutant protein has lost this localization. A loss of the cut8+ gene encoding an anchor of the proteasome to the nuclear membrane causes similar phenotypes as observed in the rpt3-1 mutant. Thus, we propose that the proteasome (or its subcomplex) associates with centromeric chromatin and regulates distribution of CENP-A.

Suggested Citation

  • Teppei Kitagawa & Kojiro Ishii & Kojiro Takeda & Tomohiro Matsumoto, 2014. "The 19S proteasome subunit Rpt3 regulates distribution of CENP-A by associating with centromeric chromatin," Nature Communications, Nature, vol. 5(1), pages 1-10, May.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms4597
    DOI: 10.1038/ncomms4597
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms4597
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms4597?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms4597. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.