IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v5y2014i1d10.1038_ncomms4562.html
   My bibliography  Save this article

Experimental orthotopic transplantation of a tissue-engineered oesophagus in rats

Author

Listed:
  • Sebastian Sjöqvist

    (Advanced Center for Translational Regenerative Medicine (ACTREM), Intervention and Technology (CLINTEC), Karolinska Institutet, SE-141 86
    Nose and Throat, Karolinska University Hospital)

  • Philipp Jungebluth

    (Advanced Center for Translational Regenerative Medicine (ACTREM), Intervention and Technology (CLINTEC), Karolinska Institutet, SE-141 86
    Nose and Throat, Karolinska University Hospital)

  • Mei Ling Lim

    (Advanced Center for Translational Regenerative Medicine (ACTREM), Intervention and Technology (CLINTEC), Karolinska Institutet, SE-141 86
    Nose and Throat, Karolinska University Hospital)

  • Johannes C. Haag

    (Advanced Center for Translational Regenerative Medicine (ACTREM), Intervention and Technology (CLINTEC), Karolinska Institutet, SE-141 86
    Nose and Throat, Karolinska University Hospital)

  • Ylva Gustafsson

    (Advanced Center for Translational Regenerative Medicine (ACTREM), Intervention and Technology (CLINTEC), Karolinska Institutet, SE-141 86
    Nose and Throat, Karolinska University Hospital)

  • Greg Lemon

    (Advanced Center for Translational Regenerative Medicine (ACTREM), Intervention and Technology (CLINTEC), Karolinska Institutet, SE-141 86)

  • Silvia Baiguera

    (Advanced Center for Translational Regenerative Medicine (ACTREM), Intervention and Technology (CLINTEC), Karolinska Institutet, SE-141 86)

  • Miguel Angel Burguillos

    (Cancer Centrum Karolinska, Karolinska Institutet)

  • Costantino Del Gaudio

    (Intrauniversitary Consortium for Material Science and Technology (INSTM) Research Unit Tor Vergata, University of Rome)

  • Antonio Beltrán Rodríguez

    (Advanced Center for Translational Regenerative Medicine (ACTREM), Intervention and Technology (CLINTEC), Karolinska Institutet, SE-141 86
    Nose and Throat, Karolinska University Hospital)

  • Alexander Sotnichenko

    (International Scientific-Research Clinical and Educational Center of Regenerative Medicine, Kuban State Medical University)

  • Karolina Kublickiene

    (Center for Gender Medicine, Karolinska Institutet
    Intervention and Technology (CLINTEC), Karolinska Institutet)

  • Henrik Ullman

    (Karolinska Institutet)

  • Heike Kielstein

    (Faculty of Medicine, Martin Luther University Halle-Wittenberg)

  • Peter Damberg

    (Intervention and Technology (CLINTEC) Karolinska Institutet)

  • Alessandra Bianco

    (Intrauniversitary Consortium for Material Science and Technology (INSTM) Research Unit Tor Vergata, University of Rome)

  • Rainer Heuchel

    (Intervention and Technology (CLINTEC), Karolinska Institutet
    Center of Biosciences, Karolinska Institutet)

  • Ying Zhao

    (Intervention and Technology (CLINTEC), Karolinska Institutet
    Center of Biosciences, Karolinska Institutet)

  • Domenico Ribatti

    (Neurosciences and Sensory Organs, University of Bari Medical School, National Cancer Institute ‘Giovanni Paolo II’)

  • Cristián Ibarra

    (Karolinska Institutet)

  • Bertrand Joseph

    (Cancer Centrum Karolinska, Karolinska Institutet)

  • Doris A. Taylor

    (Texas Heart Institute, Center for Regenerative Medicine)

  • Paolo Macchiarini

    (Advanced Center for Translational Regenerative Medicine (ACTREM), Intervention and Technology (CLINTEC), Karolinska Institutet, SE-141 86
    Nose and Throat, Karolinska University Hospital)

Abstract

A tissue-engineered oesophageal scaffold could be very useful for the treatment of pediatric and adult patients with benign or malignant diseases such as carcinomas, trauma or congenital malformations. Here we decellularize rat oesophagi inside a perfusion bioreactor to create biocompatible biological rat scaffolds that mimic native architecture, resist mechanical stress and induce angiogenesis. Seeded allogeneic mesenchymal stromal cells spontaneously differentiate (proven by gene-, protein and functional evaluations) into epithelial- and muscle-like cells. The reseeded scaffolds are used to orthotopically replace the entire cervical oesophagus in immunocompetent rats. All animals survive the 14-day study period, with patent and functional grafts, and gain significantly more weight than sham-operated animals. Explanted grafts show regeneration of all the major cell and tissue components of the oesophagus including functional epithelium, muscle fibres, nerves and vasculature. We consider the presented tissue-engineered oesophageal scaffolds a significant step towards the clinical application of bioengineered oesophagi.

Suggested Citation

  • Sebastian Sjöqvist & Philipp Jungebluth & Mei Ling Lim & Johannes C. Haag & Ylva Gustafsson & Greg Lemon & Silvia Baiguera & Miguel Angel Burguillos & Costantino Del Gaudio & Antonio Beltrán Rodríguez, 2014. "Experimental orthotopic transplantation of a tissue-engineered oesophagus in rats," Nature Communications, Nature, vol. 5(1), pages 1-15, May.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms4562
    DOI: 10.1038/ncomms4562
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms4562
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms4562?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms4562. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.