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One-step pipetting and assembly of encoded chemical-laden microparticles for high-throughput multiplexed bioassays

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  • Su Eun Chung

    (Center for Nanoparticle Research, Institute for Basic Science (IBS), Seoul National University
    Seoul National University
    Inter-university Semiconductor Research Center, Seoul National University)

  • Jiyun Kim

    (Center for Nanoparticle Research, Institute for Basic Science (IBS), Seoul National University
    Seoul National University
    Inter-university Semiconductor Research Center, Seoul National University)

  • Dong Yoon Oh

    (Center for Nanoparticle Research, Institute for Basic Science (IBS), Seoul National University
    Inter-university Semiconductor Research Center, Seoul National University
    Interdisciplinary program of Bioengineering, Seoul National University, Gwanakro 1)

  • Younghoon Song

    (Center for Nanoparticle Research, Institute for Basic Science (IBS), Seoul National University
    Seoul National University
    Inter-university Semiconductor Research Center, Seoul National University)

  • Sung Hoon Lee

    (Seoul National University)

  • Seungki Min

    (Seoul National University)

  • Sunghoon Kwon

    (Center for Nanoparticle Research, Institute for Basic Science (IBS), Seoul National University
    Seoul National University
    Inter-university Semiconductor Research Center, Seoul National University)

Abstract

One quantitative liquid handling method in conventional assay processes is pipetting, which delivers a precise volume of one sample at a time. As this process becomes laborious and time-consuming as the number of samples increases, researchers in individual laboratories need a way to conduct large-scale assays in a reasonable amount of time and at an affordable cost. Here we report a novel handling technique of chemical substances termed ‘partipetting’, which allows the one-step pipetting of various chemical-laden hydrogels. We pipette and assemble various types of encoded chemical-laden microparticles in microwell arrays in parallel. The combination of this heterogeneous particle chip and a cell chip induces the release of the chemicals from the hydrogels and, eventually, the chemicals treat the targets. Based on bioassay applications using partipetting, we show its capability in large-scale bioassays, without the need for high-throughput bioassay resources, owing to a reduction in the assay costs and time.

Suggested Citation

  • Su Eun Chung & Jiyun Kim & Dong Yoon Oh & Younghoon Song & Sung Hoon Lee & Seungki Min & Sunghoon Kwon, 2014. "One-step pipetting and assembly of encoded chemical-laden microparticles for high-throughput multiplexed bioassays," Nature Communications, Nature, vol. 5(1), pages 1-12, May.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms4468
    DOI: 10.1038/ncomms4468
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