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Temporal identity transition from Purkinje cell progenitors to GABAergic interneuron progenitors in the cerebellum

Author

Listed:
  • Yusuke Seto

    (National Institute of Neuroscience, NCNP
    Major in Integrative Bioscience and Biomedical Engineering, Faculty of Science and Engineering, Waseda University)

  • Tomoya Nakatani

    (KAN Research Institute Inc., 3F, Kobe MI R&D Center)

  • Norihisa Masuyama

    (National Institute of Neuroscience, NCNP)

  • Shinichiro Taya

    (National Institute of Neuroscience, NCNP)

  • Minoru Kumai

    (KAN Research Institute Inc., 3F, Kobe MI R&D Center)

  • Yasuko Minaki

    (KAN Research Institute Inc., 3F, Kobe MI R&D Center
    Present address: Center for iPS Cell Research and Application, Kyoto university, 53 Kawahara-cho, shogoin, Sakyo-ku, Kyoto 606-8507, Japan)

  • Akiko Hamaguchi

    (KAN Research Institute Inc., 3F, Kobe MI R&D Center)

  • Yukiko U. Inoue

    (National Institute of Neuroscience, NCNP)

  • Takayoshi Inoue

    (National Institute of Neuroscience, NCNP)

  • Satoshi Miyashita

    (National Institute of Neuroscience, NCNP
    Graduate School of Advanced Science and Engineering, Waseda University)

  • Tomoyuki Fujiyama

    (National Institute of Neuroscience, NCNP)

  • Mayumi Yamada

    (National Institute of Neuroscience, NCNP)

  • Heather Chapman

    (Cincinnati Children’s Hospital Medical Center, University of Cincinnati College of Medicine)

  • Kenneth Campbell

    (Cincinnati Children’s Hospital Medical Center, University of Cincinnati College of Medicine)

  • Mark A. Magnuson

    (Vanderbilt University School of Medicine)

  • Christopher V. Wright

    (Vanderbilt University Program in Developmental Biology, Vanderbilt University Medical Center)

  • Yoshiya Kawaguchi

    (Center for iPS Cell Research and Application, Kyoto University)

  • Kazuhiro Ikenaka

    (National Institute for Physiological Sciences
    School of Life Sciences, Graduate University for Advanced Studies)

  • Hirohide Takebayashi

    (National Institute for Physiological Sciences
    School of Life Sciences, Graduate University for Advanced Studies
    Graduate School of Medical and Dental Sciences, Niigata University)

  • Shin’ichi Ishiwata

    (Major in Integrative Bioscience and Biomedical Engineering, Faculty of Science and Engineering, Waseda University
    Waseda Bioscience Research Institute in Singapore, Waseda University)

  • Yuichi Ono

    (KAN Research Institute Inc., 3F, Kobe MI R&D Center)

  • Mikio Hoshino

    (National Institute of Neuroscience, NCNP)

Abstract

In the cerebellum, all GABAergic neurons are generated from the Ptf1a-expressing ventricular zone (Ptf1a domain). However, the machinery to produce different types of GABAergic neurons remains elusive. Here we show temporal regulation of distinct GABAergic neuron progenitors in the cerebellum. Within the Ptf1a domain at early stages, we find two subpopulations; dorsally and ventrally located progenitors that express Olig2 and Gsx1, respectively. Lineage tracing reveals the former are exclusively Purkinje cell progenitors (PCPs) and the latter Pax2-positive interneuron progenitors (PIPs). As development proceeds, PCPs gradually become PIPs starting from ventral to dorsal. In gain- and loss-of-function mutants for Gsx1 and Olig1/2, we observe abnormal transitioning from PCPs to PIPs at inappropriate developmental stages. Our findings suggest that the temporal identity transition of cerebellar GABAergic neuron progenitors from PCPs to PIPs is negatively regulated by Olig2 and positively by Gsx1, and contributes to understanding temporal control of neuronal progenitor identities.

Suggested Citation

  • Yusuke Seto & Tomoya Nakatani & Norihisa Masuyama & Shinichiro Taya & Minoru Kumai & Yasuko Minaki & Akiko Hamaguchi & Yukiko U. Inoue & Takayoshi Inoue & Satoshi Miyashita & Tomoyuki Fujiyama & Mayum, 2014. "Temporal identity transition from Purkinje cell progenitors to GABAergic interneuron progenitors in the cerebellum," Nature Communications, Nature, vol. 5(1), pages 1-13, May.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms4337
    DOI: 10.1038/ncomms4337
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