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A sensitive two-photon probe to selectively detect monoamine oxidase B activity in Parkinson’s disease models

Author

Listed:
  • Lin Li

    (National University of Singapore)

  • Cheng-Wu Zhang

    (National Neuroscience Institute)

  • Grace Y. J. Chen

    (National University of Singapore)

  • Biwei Zhu

    (National University of Singapore)

  • Chou Chai

    (Duke-NUS Graduate Medical School)

  • Qing-Hua Xu

    (National University of Singapore)

  • Eng-King Tan

    (National Neuroscience Institute)

  • Qing Zhu

    (Institute of Bioengineering, Zhejiang University of Technology)

  • Kah-Leong Lim

    (National Neuroscience Institute
    Duke-NUS Graduate Medical School
    National University of Singapore)

  • Shao Q. Yao

    (National University of Singapore)

Abstract

The unusually high MAO-B activity consistently observed in Parkinson’s disease (PD) patients has been proposed as a biomarker; however, this has not been realized due to the lack of probes suitable for MAO-B-specific detection in live cells/tissues. Here we report the first two-photon, small molecule fluorogenic probe (U1) that enables highly sensitive/specific and real-time imaging of endogenous MAO-B activities across biological samples. We also used U1 to confirm the reported inverse relationship between parkin and MAO-B in PD models. With no apparent toxicity, U1 may be used to monitor MAO-B activities in small animals during disease development. In clinical samples, we find elevated MAO-B activities only in B lymphocytes (not in fibroblasts), hinting that MAO-B activity in peripheral blood cells might be an accessible biomarker for rapid detection of PD. Our results provide important starting points for using small molecule imaging techniques to explore MAO-B at the organism level.

Suggested Citation

  • Lin Li & Cheng-Wu Zhang & Grace Y. J. Chen & Biwei Zhu & Chou Chai & Qing-Hua Xu & Eng-King Tan & Qing Zhu & Kah-Leong Lim & Shao Q. Yao, 2014. "A sensitive two-photon probe to selectively detect monoamine oxidase B activity in Parkinson’s disease models," Nature Communications, Nature, vol. 5(1), pages 1-10, May.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms4276
    DOI: 10.1038/ncomms4276
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