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Generation of folliculogenic human epithelial stem cells from induced pluripotent stem cells

Author

Listed:
  • Ruifeng Yang

    (Perelman School of Medicine, University of Pennsylvania)

  • Ying Zheng

    (Kligman Laboratories, Perelman School of Medicine, University of Pennsylvania)

  • Michelle Burrows

    (Kligman Laboratories, Perelman School of Medicine, University of Pennsylvania)

  • Shujing Liu

    (Perelman School of Medicine, University of Pennsylvania)

  • Zhi Wei

    (New Jersey Institute of Technology)

  • Arben Nace

    (Kligman Laboratories, Perelman School of Medicine, University of Pennsylvania)

  • Wei Guo

    (University of Pennsylvania)

  • Suresh Kumar

    (Perelman School of Medicine, University of Pennsylvania)

  • George Cotsarelis

    (Kligman Laboratories, Perelman School of Medicine, University of Pennsylvania)

  • Xiaowei Xu

    (Perelman School of Medicine, University of Pennsylvania)

Abstract

Epithelial stem cells (EpSCs) in the hair follicle bulge are required for hair follicle growth and cycling. The isolation and propagation of human EpSCs for tissue engineering purposes remains a challenge. Here we develop a strategy to differentiate human iPSCs (hiPSCs) into CD200+/ITGA6+ EpSCs that can reconstitute the epithelial components of the hair follicle and interfollicular epidermis. The hiPSC-derived CD200+/ITGA6+ cells show a similar gene expression signature as EpSCs directly isolated from human hair follicles. Human iPSC-derived CD200+/ITGA6+ cells are capable of generating all hair follicle lineages including the hair shaft, and the inner and outer root sheaths in skin reconstitution assays. The regenerated hair follicles possess a KRT15+ stem cell population and produce hair shafts expressing hair-specific keratins. These results suggest an approach for generating large numbers of human EpSCs for tissue engineering and new treatments for hair loss, wound healing and other degenerative skin disorders.

Suggested Citation

  • Ruifeng Yang & Ying Zheng & Michelle Burrows & Shujing Liu & Zhi Wei & Arben Nace & Wei Guo & Suresh Kumar & George Cotsarelis & Xiaowei Xu, 2014. "Generation of folliculogenic human epithelial stem cells from induced pluripotent stem cells," Nature Communications, Nature, vol. 5(1), pages 1-11, May.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms4071
    DOI: 10.1038/ncomms4071
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