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Familial Alzheimer’s mutations within APPTM increase Aβ42 production by enhancing accessibility of ε-cleavage site

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  • Wen Chen

    (Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute)

  • Eric Gamache

    (Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute)

  • David J. Rosenman

    (Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute)

  • Jian Xie

    (Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute)

  • Maria M. Lopez

    (Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute)

  • Yue-Ming Li

    (Molecular Pharmacology and Chemistry Program, Memorial Sloan-Kettering Cancer Center)

  • Chunyu Wang

    (Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute)

Abstract

The high Aβ42/Aβ40 production ratio is a hallmark of familial Alzheimer’s disease, which can be caused by mutations in the amyloid precursor protein (APP). The C-terminus of Aβ is generated by γ-secretase cleavage within the transmembrane domain of APP (APPTM), a process that is primed by an initial ε-cleavage at either T48 or L49, resulting in subsequent production of Aβ42 or Aβ40, respectively. Here we solve the dimer structures of wild-type APPTM (AAPTM WT) and mutant APPTM (FAD mutants V44M) with solution NMR. The right-handed APPTM helical dimer is mediated by GXXXA motif. From the NMR structural and dynamic data, we show that the V44M and V44A mutations can selectively expose the T48 site by weakening helical hydrogen bonds and increasing hydrogen–deuterium exchange rate (kex). We propose a structural model in which FAD mutations (V44M and V44A) can open the T48 site γ-secretase for the initial ε-cleavage, and consequently shift cleavage preference towards Aβ42.

Suggested Citation

  • Wen Chen & Eric Gamache & David J. Rosenman & Jian Xie & Maria M. Lopez & Yue-Ming Li & Chunyu Wang, 2014. "Familial Alzheimer’s mutations within APPTM increase Aβ42 production by enhancing accessibility of ε-cleavage site," Nature Communications, Nature, vol. 5(1), pages 1-7, May.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms4037
    DOI: 10.1038/ncomms4037
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