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Structure of a photosynthetic reaction centre determined by serial femtosecond crystallography

Author

Listed:
  • Linda C. Johansson

    (University of Gothenburg)

  • David Arnlund

    (University of Gothenburg)

  • Gergely Katona

    (University of Gothenburg)

  • Thomas A. White

    (Center for Free-Electron Laser Science, DESY)

  • Anton Barty

    (Center for Free-Electron Laser Science, DESY)

  • Daniel P. DePonte

    (Center for Free-Electron Laser Science, DESY)

  • Robert L. Shoeman

    (Max-Planck-Institut für Medizinische Forschung
    Max Planck Advanced Study Group, Center for Free-Electron Laser Science)

  • Cecilia Wickstrand

    (University of Gothenburg)

  • Amit Sharma

    (University of Gothenburg)

  • Garth J. Williams

    (Linac Coherent Light Source, LCLS, SLAC National Accelerator Laboratory)

  • Andrew Aquila

    (Center for Free-Electron Laser Science, DESY)

  • Michael J. Bogan

    (PULSE Institute, SLAC National Accelerator Laboratory)

  • Carl Caleman

    (Center for Free-Electron Laser Science, DESY)

  • Jan Davidsson

    (Uppsala University)

  • R Bruce Doak

    (Arizona State University)

  • Matthias Frank

    (Lawrence Livermore National Laboratory)

  • Raimund Fromme

    (Arizona State University)

  • Lorenzo Galli

    (Center for Free-Electron Laser Science, DESY
    University of Hamburg)

  • Ingo Grotjohann

    (Arizona State University)

  • Mark S. Hunter

    (Arizona State University)

  • Stephan Kassemeyer

    (Max-Planck-Institut für Medizinische Forschung
    Max Planck Advanced Study Group, Center for Free-Electron Laser Science)

  • Richard A. Kirian

    (Arizona State University)

  • Christopher Kupitz

    (Arizona State University)

  • Mengning Liang

    (Center for Free-Electron Laser Science, DESY)

  • Lukas Lomb

    (Max-Planck-Institut für Medizinische Forschung
    Max Planck Advanced Study Group, Center for Free-Electron Laser Science)

  • Erik Malmerberg

    (University of Gothenburg)

  • Andrew V. Martin

    (Center for Free-Electron Laser Science, DESY)

  • Marc Messerschmidt

    (Linac Coherent Light Source, LCLS, SLAC National Accelerator Laboratory)

  • Karol Nass

    (Center for Free-Electron Laser Science, DESY
    University of Hamburg)

  • Lars Redecke

    (Joint Laboratory for Structural Biology of Infection and Inflammation, Institute of Biochemistry and Molecular Biology, University of Hamburg and Institute of Biochemistry, University of Lübeck at DESY)

  • M Marvin Seibert

    (Linac Coherent Light Source, LCLS, SLAC National Accelerator Laboratory)

  • Jennie Sjöhamn

    (University of Gothenburg)

  • Jan Steinbrener

    (Max-Planck-Institut für Medizinische Forschung
    Max Planck Advanced Study Group, Center for Free-Electron Laser Science)

  • Francesco Stellato

    (Center for Free-Electron Laser Science, DESY)

  • Dingjie Wang

    (Arizona State University)

  • Weixaio Y. Wahlgren

    (University of Gothenburg)

  • Uwe Weierstall

    (Arizona State University)

  • Sebastian Westenhoff

    (University of Gothenburg)

  • Nadia A. Zatsepin

    (Arizona State University)

  • Sébastien Boutet

    (Linac Coherent Light Source, LCLS, SLAC National Accelerator Laboratory)

  • John C.H. Spence

    (Arizona State University)

  • Ilme Schlichting

    (Max-Planck-Institut für Medizinische Forschung
    Max Planck Advanced Study Group, Center for Free-Electron Laser Science)

  • Henry N. Chapman

    (Center for Free-Electron Laser Science, DESY
    University of Hamburg)

  • Petra Fromme

    (Arizona State University)

  • Richard Neutze

    (University of Gothenburg)

Abstract

Serial femtosecond crystallography is an X-ray free-electron-laser-based method with considerable potential to have an impact on challenging problems in structural biology. Here we present X-ray diffraction data recorded from microcrystals of the Blastochloris viridis photosynthetic reaction centre to 2.8 Å resolution and determine its serial femtosecond crystallography structure to 3.5 Å resolution. Although every microcrystal is exposed to a dose of 33 MGy, no signs of X-ray-induced radiation damage are visible in this integral membrane protein structure.

Suggested Citation

  • Linda C. Johansson & David Arnlund & Gergely Katona & Thomas A. White & Anton Barty & Daniel P. DePonte & Robert L. Shoeman & Cecilia Wickstrand & Amit Sharma & Garth J. Williams & Andrew Aquila & Mic, 2013. "Structure of a photosynthetic reaction centre determined by serial femtosecond crystallography," Nature Communications, Nature, vol. 4(1), pages 1-7, December.
    • Handle: RePEc:nat:natcom:v:4:y:2013:i:1:d:10.1038_ncomms3911
    DOI: 10.1038/ncomms3911
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