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miRNomes of haematopoietic stem cells and dendritic cells identify miR-30b as a regulator of Notch1

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  • Xiaoping Su

    (National Key Laboratory of Medical Immunology and Institute of Immunology, Second Military Medical University)

  • Cheng Qian

    (National Key Laboratory of Medical Immunology and Institute of Immunology, Second Military Medical University)

  • Qian Zhang

    (Chinese Academy of Medical Sciences)

  • Jin Hou

    (National Key Laboratory of Medical Immunology and Institute of Immunology, Second Military Medical University)

  • Yan Gu

    (National Key Laboratory of Medical Immunology and Institute of Immunology, Second Military Medical University)

  • Yanmei Han

    (National Key Laboratory of Medical Immunology and Institute of Immunology, Second Military Medical University)

  • Yongjian Chen

    (National Key Laboratory of Medical Immunology and Institute of Immunology, Second Military Medical University)

  • Minghong Jiang

    (Chinese Academy of Medical Sciences)

  • Xuetao Cao

    (National Key Laboratory of Medical Immunology and Institute of Immunology, Second Military Medical University
    Chinese Academy of Medical Sciences)

Abstract

Dendritic cells (DCs) are critical to initiate the immune response and maintain tolerance, depending on different status and subsets. The expression profiles of microRNAs (miRNAs) in various DC subsets and haematopoietic stem cells (HSCs), which generate DCs, remain to be fully identified. Here we examine miRNomes of mouse bone marrow HSCs, immature DCs, mature DCs and IL-10/NO-producing regulatory DCs by deep sequencing. We identify numerous stage-specific miRNAs and histone modification in HSCs and DCs at different differentiation stages. miR-30b, significantly upregulated via a TGF-beta/Smad3-mediated epigenetic pathway in regulatory DCs, can target Notch1 to promote IL-10 and NO production, suggesting that miR-30b is a negative regulator of immune response. We also identify miRNomes of in vivo counterparts of mature DCs and regulatory DCs and systematically compare them with DCs cultured in vitro. These results provide a resource for studying roles of miRNAs in stem cell biology, development and functional regulation of DC subsets.

Suggested Citation

  • Xiaoping Su & Cheng Qian & Qian Zhang & Jin Hou & Yan Gu & Yanmei Han & Yongjian Chen & Minghong Jiang & Xuetao Cao, 2013. "miRNomes of haematopoietic stem cells and dendritic cells identify miR-30b as a regulator of Notch1," Nature Communications, Nature, vol. 4(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:4:y:2013:i:1:d:10.1038_ncomms3903
    DOI: 10.1038/ncomms3903
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