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DNA-based highly tunable particle focuser

Author

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  • Kyowon Kang

    (Ajou University
    Ajou University)

  • Sung Sik Lee

    (Institute of Biochemistry, ETH Zurich)

  • Kyu Hyun

    (School of Chemical and Biomolecular Engineering, Pusan National University)

  • Seong Jae Lee

    (The University of Suwon, Hwaseong)

  • Ju Min Kim

    (Ajou University
    Ajou University)

Abstract

DNA is distinguished by both long length and structural rigidity. Classical polymer theories predict that DNA enhances the non-Newtonian elastic properties of its dilute solution more significantly than common synthetic flexible polymers because of its larger size and longer relaxation time. Here we exploit this property to report that under Poiseuille microflow, rigid spherical particles laterally migrate and form a tightly focused stream in an extremely dilute DNA solution (0.0005 (w/v)%). By the use of the DNA solution, we achieve highly efficient focusing (>99.5%) over an unprecedented wide range of flow rates (ratio of maximum to minimum flow rates ~400). This highly tunable particle-focusing technique can be used in the design of cost-effective portable flow cytometers, high-throughput cell analysis and also for cell sorting by size. We demonstrate that DNA is an efficient elasticity enhancer, which originates from its unique structural properties.

Suggested Citation

  • Kyowon Kang & Sung Sik Lee & Kyu Hyun & Seong Jae Lee & Ju Min Kim, 2013. "DNA-based highly tunable particle focuser," Nature Communications, Nature, vol. 4(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:4:y:2013:i:1:d:10.1038_ncomms3567
    DOI: 10.1038/ncomms3567
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