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In vivo imaging and histochemistry are combined in the cryosection labelling and intravital microscopy technique

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  • Laila Ritsma

    (Hubrecht Institute-Royal Dutch Academy of Science and University Medical Center Utrecht)

  • Nienke Vrisekoop

    (Hubrecht Institute-Royal Dutch Academy of Science and University Medical Center Utrecht)

  • Jacco van Rheenen

    (Hubrecht Institute-Royal Dutch Academy of Science and University Medical Center Utrecht)

Abstract

Intravital microscopy has been used extensively to study dynamic processes in the context of a living animal; however, only a limited number of fluorescent probes and mouse models are available. By contrast, many dyes and antibodies exist for the immuno-labelling of fixed tissue. Here we report a method that combines the advantages of histochemistry and in vivo imaging by correlating cryosection labelling with corresponding intravital microscopy images (CLIM). Using CLIM, we find that the presence of CD3+ T cells correlates with mammary tumour cell migration. When CD4+ and CD8+ T-cell subsets are depleted, reduced tumour cell migration is observed. From these data we conclude that CLIM is a powerful tool to correlate intravital microscopy data with cryosection labelling data.

Suggested Citation

  • Laila Ritsma & Nienke Vrisekoop & Jacco van Rheenen, 2013. "In vivo imaging and histochemistry are combined in the cryosection labelling and intravital microscopy technique," Nature Communications, Nature, vol. 4(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:4:y:2013:i:1:d:10.1038_ncomms3366
    DOI: 10.1038/ncomms3366
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