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In vivo time-gated fluorescence imaging with biodegradable luminescent porous silicon nanoparticles

Author

Listed:
  • Luo Gu

    (University of California, San Diego
    School of Engineering and Applied Sciences, Harvard University
    Wyss Institute for Biologically Inspired Engineering, Harvard University)

  • David J. Hall

    (University of California, San Diego
    Moores Cancer Center, University of California, San Diego)

  • Zhengtao Qin

    (University of California, San Diego)

  • Emily Anglin

    (University of California, San Diego)

  • Jinmyoung Joo

    (University of California, San Diego)

  • David J. Mooney

    (School of Engineering and Applied Sciences, Harvard University
    Wyss Institute for Biologically Inspired Engineering, Harvard University)

  • Stephen B. Howell

    (Moores Cancer Center, University of California, San Diego
    University of California, San Diego)

  • Michael J. Sailor

    (University of California, San Diego
    Moores Cancer Center, University of California, San Diego)

Abstract

Fluorescence imaging is one of the most versatile and widely used visualization methods in biomedical research. However, tissue autofluorescence is a major obstacle confounding interpretation of in vivo fluorescence images. The unusually long emission lifetime (5–13 μs) of photoluminescent porous silicon nanoparticles can allow the time-gated imaging of tissues in vivo, completely eliminating shorter-lived ( 50-fold in vitro and by >20-fold in vivo when imaging porous silicon nanoparticles. Time-gated imaging of porous silicon nanoparticles accumulated in a human ovarian cancer xenograft following intravenous injection is demonstrated in a live mouse. The potential for multiplexing of images in the time domain by using separate porous silicon nanoparticles engineered with different excited state lifetimes is discussed.

Suggested Citation

  • Luo Gu & David J. Hall & Zhengtao Qin & Emily Anglin & Jinmyoung Joo & David J. Mooney & Stephen B. Howell & Michael J. Sailor, 2013. "In vivo time-gated fluorescence imaging with biodegradable luminescent porous silicon nanoparticles," Nature Communications, Nature, vol. 4(1), pages 1-7, October.
    • Handle: RePEc:nat:natcom:v:4:y:2013:i:1:d:10.1038_ncomms3326
    DOI: 10.1038/ncomms3326
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