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Srs2 prevents Rad51 filament formation by repetitive motion on DNA

Author

Listed:
  • Yupeng Qiu

    (University of Illinois at Urbana-Champaign)

  • Edwin Antony

    (Utah State University)

  • Sultan Doganay

    (Institute for Genomic Biology, University of Illinois at Urbana-Champaign
    Center for Biophysics and Computational Biology, University of Illinois at Urbana-Champaign)

  • Hye Ran Koh

    (Institute for Genomic Biology, University of Illinois at Urbana-Champaign
    University of Illinois at Urbana-Champaign)

  • Timothy M. Lohman

    (Washington University School of Medicine)

  • Sua Myong

    (University of Illinois at Urbana-Champaign
    Institute for Genomic Biology, University of Illinois at Urbana-Champaign
    Center for Biophysics and Computational Biology, University of Illinois at Urbana-Champaign
    Center of Physics for Living Cells, University of Illinois at Urbana-Champaign)

Abstract

Srs2 dismantles presynaptic Rad51 filaments and prevents its re-formation as an anti-recombinase. However, the molecular mechanism by which Srs2 accomplishes these tasks remains unclear. Here we report a single-molecule fluorescence study of the dynamics of Rad51 filament formation and its disruption by Srs2. Rad51 forms filaments on single-stranded DNA by sequential binding of primarily monomers and dimers in a 5′–3′ direction. One Rad51 molecule binds to three nucleotides, and six monomers are required to achieve a stable nucleation cluster. Srs2 exhibits ATP-dependent repetitive motion on single-stranded DNA and this activity prevents re-formation of the Rad51 filament. The same activity of Srs2 cannot prevent RecA filament formation, indicating its specificity for Rad51. Srs2’s DNA-unwinding activity is greatly suppressed when Rad51 filaments form on duplex DNA. Taken together, our results reveal an exquisite and highly specific mechanism by which Srs2 regulates the Rad51 filament formation.

Suggested Citation

  • Yupeng Qiu & Edwin Antony & Sultan Doganay & Hye Ran Koh & Timothy M. Lohman & Sua Myong, 2013. "Srs2 prevents Rad51 filament formation by repetitive motion on DNA," Nature Communications, Nature, vol. 4(1), pages 1-10, October.
    • Handle: RePEc:nat:natcom:v:4:y:2013:i:1:d:10.1038_ncomms3281
    DOI: 10.1038/ncomms3281
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