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Endonuclease V cleaves at inosines in RNA

Author

Listed:
  • Erik Sebastian Vik

    (Oslo University Hospital HF and University of Oslo
    Oslo University Hospital HF and University of Oslo)

  • Meh Sameen Nawaz

    (Oslo University Hospital HF and University of Oslo)

  • Pernille Strøm Andersen

    (Oslo University Hospital HF and University of Oslo
    Oslo University Hospital HF and University of Oslo)

  • Cathrine Fladeby

    (Oslo University Hospital HF and University of Oslo)

  • Magnar Bjørås

    (Oslo University Hospital HF and University of Oslo
    Oslo University Hospital HF and University of Oslo)

  • Bjørn Dalhus

    (Oslo University Hospital HF and University of Oslo
    Oslo University Hospital HF and University of Oslo)

  • Ingrun Alseth

    (Oslo University Hospital HF and University of Oslo)

Abstract

Endonuclease V orthologues are highly conserved proteins found in all kingdoms of life. While the prokaryotic enzymes are DNA repair proteins for removal of deaminated adenosine (inosine) from the genome, no clear role for the eukaryotic counterparts has hitherto been described. Here we report that human endonuclease V (ENDOV) and also Escherichia coli endonuclease V are highly active ribonucleases specific for inosine in RNA. Inosines are normal residues in certain RNAs introduced by specific deaminases. Adenosine-to-inosine editing is essential for proper function of these transcripts and defects are linked to various human disease. Here we show that human ENDOV cleaves an RNA substrate containing inosine in a position corresponding to a biologically important site for deamination in the Gabra-3 transcript of the GABAA neurotransmitter. Further, human ENDOV specifically incises transfer RNAs with inosine in the wobble position. This previously unknown RNA incision activity may suggest a role for endonuclease V in normal RNA metabolism.

Suggested Citation

  • Erik Sebastian Vik & Meh Sameen Nawaz & Pernille Strøm Andersen & Cathrine Fladeby & Magnar Bjørås & Bjørn Dalhus & Ingrun Alseth, 2013. "Endonuclease V cleaves at inosines in RNA," Nature Communications, Nature, vol. 4(1), pages 1-7, October.
    • Handle: RePEc:nat:natcom:v:4:y:2013:i:1:d:10.1038_ncomms3271
    DOI: 10.1038/ncomms3271
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