IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v4y2013i1d10.1038_ncomms3247.html
   My bibliography  Save this article

A poly(A)-specific ribonuclease directly regulates the poly(A) status of mitochondrial mRNA in Arabidopsis

Author

Listed:
  • Takashi Hirayama

    (Institute of Plant Science and Resources, Okayama University)

  • Takakazu Matsuura

    (Institute of Plant Science and Resources, Okayama University)

  • Sho Ushiyama

    (Graduate School of Nanobioscience, Yokohama City University)

  • Mari Narusaka

    (Research Institute for Biological Sciences Okayama)

  • Yukio Kurihara

    (Plant Science Center, RIKEN)

  • Michiko Yasuda

    (RIKEN Innovation Center)

  • Misato Ohtani

    (Biomass Engineering Program, RIKEN)

  • Motoaki Seki

    (Plant Science Center, RIKEN)

  • Taku Demura

    (Biomass Engineering Program, RIKEN)

  • Hideo Nakashita

    (RIKEN Innovation Center)

  • Yoshihiro Narusaka

    (Research Institute for Biological Sciences Okayama)

  • Shimpei Hayashi

    (Graduate School of Nanobioscience, Yokohama City University)

Abstract

Coordination of gene expression in the organelles and the nucleus is important for eukaryotic cell function. Transcriptional and post-transcriptional gene regulation in mitochondria remains incompletely understood in most eukaryotes, including plants. Here we show that poly(A)-specific ribonuclease, which influences the poly(A) status of cytoplasmic mRNA in many eukaryotes, directly regulates the poly(A) tract of mitochondrial mRNA in conjunction with a bacterial-type poly(A) polymerase, AGS1, in Arabidopsis. An Arabidopsis poly(A)-specific ribonuclease-deficient mutant, ahg2-1, accumulates polyadenylated mitochondrial mRNA and shows defects in mitochondrial protein complex levels. Mutations of AGS1 suppress the ahg2-1 phenotype. Mitochondrial localizations of AHG2 and AGS1 are required for their functions in the regulation of the poly(A) tract of mitochondrial mRNA. Our findings suggest that AHG2 and AGS1 constitute a regulatory system that controls mitochondrial mRNA poly(A) status in Arabidopsis.

Suggested Citation

  • Takashi Hirayama & Takakazu Matsuura & Sho Ushiyama & Mari Narusaka & Yukio Kurihara & Michiko Yasuda & Misato Ohtani & Motoaki Seki & Taku Demura & Hideo Nakashita & Yoshihiro Narusaka & Shimpei Haya, 2013. "A poly(A)-specific ribonuclease directly regulates the poly(A) status of mitochondrial mRNA in Arabidopsis," Nature Communications, Nature, vol. 4(1), pages 1-9, October.
    • Handle: RePEc:nat:natcom:v:4:y:2013:i:1:d:10.1038_ncomms3247
    DOI: 10.1038/ncomms3247
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms3247
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms3247?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:4:y:2013:i:1:d:10.1038_ncomms3247. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.