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Hyperthin nanochains composed of self-polymerizing protein shackles

Author

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  • Ryo Matsunaga

    (The Medical Proteomics Laboratory, The Institute of Medical Science, The University of Tokyo
    Graduate School of Frontier Sciences, The University of Tokyo)

  • Saeko Yanaka

    (The Medical Proteomics Laboratory, The Institute of Medical Science, The University of Tokyo
    Graduate School of Frontier Sciences, The University of Tokyo)

  • Satoru Nagatoishi

    (The Medical Proteomics Laboratory, The Institute of Medical Science, The University of Tokyo
    Graduate School of Engineering, The University of Tokyo)

  • Kouhei Tsumoto

    (The Medical Proteomics Laboratory, The Institute of Medical Science, The University of Tokyo
    Graduate School of Frontier Sciences, The University of Tokyo
    Graduate School of Engineering, The University of Tokyo
    Graduate School of Engineering, The University of Tokyo)

Abstract

Protein fibrils are expected to have applications as functional nanomaterials because of their sophisticated structures; however, nanoscale ordering of the functional units of protein fibrils remains challenging. Here we design a series of self-polymerizing protein monomers, referred to as protein shackles, derived from modified recombinant subunits of pili from Streptococcus pyogenes. The monomers polymerize into nanochains through spontaneous irreversible covalent bond formation. We design the protein shackles so that their reactions can be controlled by altering redox conditions, which affect disulphide bond formation between engineered cysteine residues. The interaction between the monomers improves their polymerization reactivity and determines morphologies of the polymers. In addition, green fluorescent protein-tagged protein shackles can polymerize, indicating proteins can be stably attached to the nanochains with its functionality preserved. Furthermore we demonstrate that a molecular-recognizable nanochain binds to its partner with an enhanced binding ability in solution. These characteristics are expected to be applied for novel protein nanomaterials.

Suggested Citation

  • Ryo Matsunaga & Saeko Yanaka & Satoru Nagatoishi & Kouhei Tsumoto, 2013. "Hyperthin nanochains composed of self-polymerizing protein shackles," Nature Communications, Nature, vol. 4(1), pages 1-10, October.
    • Handle: RePEc:nat:natcom:v:4:y:2013:i:1:d:10.1038_ncomms3211
    DOI: 10.1038/ncomms3211
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    Cited by:

    1. Claudia L. Driscoll & Anthony H. Keeble & Mark R. Howarth, 2024. "SpyMask enables combinatorial assembly of bispecific binders," Nature Communications, Nature, vol. 15(1), pages 1-17, December.
    2. Susan K. Vester & Rolle Rahikainen & Irsyad N. A. Khairil Anuar & Rory A. Hills & Tiong Kit Tan & Mark Howarth, 2022. "SpySwitch enables pH- or heat-responsive capture and release for plug-and-display nanoassembly," Nature Communications, Nature, vol. 13(1), pages 1-16, December.

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