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Phosphorylation of BLUS1 kinase by phototropins is a primary step in stomatal opening

Author

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  • Atsushi Takemiya

    (Faculty of Science, Kyushu University)

  • Naoyuki Sugiyama

    (Institute for Advanced Biosciences, Keio University
    Present address: Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan)

  • Hiroshi Fujimoto

    (Faculty of Science, Kyushu University)

  • Toshifumi Tsutsumi

    (Faculty of Science, Kyushu University)

  • Shota Yamauchi

    (Faculty of Science, Kyushu University)

  • Asami Hiyama

    (Faculty of Science, Kyushu University)

  • Yasuomi Tada

    (Life Science Research Center, Institute of Research Promotion, Kagawa University)

  • John M. Christie

    (Institute of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, University of Glasgow)

  • Ken-ichiro Shimazaki

    (Faculty of Science, Kyushu University)

Abstract

Opening of stomata in the plant facilitates photosynthetic CO2 fixation and transpiration. Blue-light perception by phototropins (phot1, phot2) activates the plasma membrane H+-ATPase, causing stomata to open. Here we describe a regulator that connects these components, a Ser/Thr protein kinase, BLUS1 (BLU E LIGHT S IGNALING 1), which mediates a primary step for phototropin signalling in guard cells. blus1 mutants identified by infrared thermography result in a loss of blue light-dependent stomatal opening. BLUS1 encodes a protein kinase that is directly phosphorylated by phot1 in vitro and in vivo at Ser-348 within its C-terminus. Both phosphorylation of Ser-348 and BLUS1 kinase activity are essential for activation of the H+-ATPase. blus1 mutants show lower stomatal conductance and CO2 assimilation than wild-type plants under decreased ambient CO2. Together, our analyses demonstrate that BLUS1 functions as a phototropin substrate and primary regulator of stomatal control to enhance photosynthetic CO2 assimilation under natural light conditions.

Suggested Citation

  • Atsushi Takemiya & Naoyuki Sugiyama & Hiroshi Fujimoto & Toshifumi Tsutsumi & Shota Yamauchi & Asami Hiyama & Yasuomi Tada & John M. Christie & Ken-ichiro Shimazaki, 2013. "Phosphorylation of BLUS1 kinase by phototropins is a primary step in stomatal opening," Nature Communications, Nature, vol. 4(1), pages 1-8, October.
    • Handle: RePEc:nat:natcom:v:4:y:2013:i:1:d:10.1038_ncomms3094
    DOI: 10.1038/ncomms3094
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