IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v4y2013i1d10.1038_ncomms2853.html
   My bibliography  Save this article

InVERT molding for scalable control of tissue microarchitecture

Author

Listed:
  • K. R. Stevens

    (Harvard-MIT Health Sciences and Technology, Institute for Medical Engineering and Science, Massachusetts Institute of Technology)

  • M. D. Ungrin

    (Institute of Biomaterials and Biomedical Engineering, University of Toronto
    The Donnelly Centre for Cellular and Biomolecular Research, University of Toronto
    McEwen Centre for Regenerative Medicine, University Health Network
    Present address: Department of Comparative Biology & Experimental Medicine, University of Calgary, Calgary, Alberta, Canada T2N 4N1)

  • R. E. Schwartz

    (Harvard-MIT Health Sciences and Technology, Institute for Medical Engineering and Science, Massachusetts Institute of Technology
    Brigham and Women’s Hospital)

  • S. Ng

    (Massachusetts Institute of Technology)

  • B. Carvalho

    (Massachusetts Institute of Technology)

  • K. S. Christine

    (Harvard-MIT Health Sciences and Technology, Institute for Medical Engineering and Science, Massachusetts Institute of Technology)

  • R. R. Chaturvedi

    (University of Pennsylvania)

  • C. Y. Li

    (Massachusetts Institute of Technology)

  • P. W. Zandstra

    (Institute of Biomaterials and Biomedical Engineering, University of Toronto
    The Donnelly Centre for Cellular and Biomolecular Research, University of Toronto
    McEwen Centre for Regenerative Medicine, University Health Network
    Heart & Stroke Richard Lewar Centre of Excellence, University of Toronto)

  • C. S. Chen

    (University of Pennsylvania)

  • S. N. Bhatia

    (Harvard-MIT Health Sciences and Technology, Institute for Medical Engineering and Science, Massachusetts Institute of Technology
    Howard Hughes Medical Institute
    Massachusetts Institute of Technology
    David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology)

Abstract

Complex tissues contain multiple cell types that are hierarchically organized within morphologically and functionally distinct compartments. Construction of engineered tissues with optimized tissue architecture has been limited by tissue fabrication techniques, which do not enable versatile microscale organization of multiple cell types in tissues of size adequate for physiological studies and tissue therapies. Here we present an ‘Intaglio-Void/Embed-Relief Topographic molding’ method for microscale organization of many cell types, including induced pluripotent stem cell-derived progeny, within a variety of synthetic and natural extracellular matrices and across tissues of sizes appropriate for in vitro, pre-clinical, and clinical studies. We demonstrate that compartmental placement of non-parenchymal cells relative to primary or induced pluripotent stem cell-derived hepatocytes, compartment microstructure, and cellular composition modulate hepatic functions. Configurations found to sustain physiological function in vitro also result in survival and function in mice for at least 4 weeks, demonstrating the importance of architectural optimization before implantation.

Suggested Citation

  • K. R. Stevens & M. D. Ungrin & R. E. Schwartz & S. Ng & B. Carvalho & K. S. Christine & R. R. Chaturvedi & C. Y. Li & P. W. Zandstra & C. S. Chen & S. N. Bhatia, 2013. "InVERT molding for scalable control of tissue microarchitecture," Nature Communications, Nature, vol. 4(1), pages 1-11, October.
    • Handle: RePEc:nat:natcom:v:4:y:2013:i:1:d:10.1038_ncomms2853
    DOI: 10.1038/ncomms2853
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms2853
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms2853?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:4:y:2013:i:1:d:10.1038_ncomms2853. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.