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Ribosomal protein S1 functions as a termination factor in RNA synthesis by Qβ phage replicase

Author

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  • Nikita N. Vasilyev

    (Institute of Protein Research of the Russian Academy of Sciences
    Present address: New York University School of Medicine, New York, New York 10016, USA)

  • Zarina S. Kutlubaeva

    (Institute of Protein Research of the Russian Academy of Sciences)

  • Victor I. Ugarov

    (Institute of Protein Research of the Russian Academy of Sciences)

  • Helena V. Chetverina

    (Institute of Protein Research of the Russian Academy of Sciences)

  • Alexander B. Chetverin

    (Institute of Protein Research of the Russian Academy of Sciences)

Abstract

S1 is the largest ribosomal protein, and is vitally important for the cell. S1 is also a subunit of Qβ replicase, the RNA-directed RNA polymerase of bacteriophage Qβ. In both protein and RNA syntheses, S1 is commonly believed to bind to a template RNA at the initiation step, and not to be involved in later events. Here, we show that in Qβ replicase-mediated RNA synthesis, S1 functions at the termination step by promoting release of the product strand in a single-stranded form. This function is fulfilled by the N-terminal fragment comprising the first two S1 domains. The results suggest that S1 might also have a role other than mRNA binding in the ribosome.

Suggested Citation

  • Nikita N. Vasilyev & Zarina S. Kutlubaeva & Victor I. Ugarov & Helena V. Chetverina & Alexander B. Chetverin, 2013. "Ribosomal protein S1 functions as a termination factor in RNA synthesis by Qβ phage replicase," Nature Communications, Nature, vol. 4(1), pages 1-7, June.
  • Handle: RePEc:nat:natcom:v:4:y:2013:i:1:d:10.1038_ncomms2807
    DOI: 10.1038/ncomms2807
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