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Magnetic barcode assay for genetic detection of pathogens

Author

Listed:
  • Monty Liong

    (Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School)

  • Anh N. Hoang

    (BioMEMS Resource Center, Surgical Services, Massachusetts General Hospital, Harvard Medical School)

  • Jaehoon Chung

    (Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School)

  • Nil Gural

    (BioMEMS Resource Center, Surgical Services, Massachusetts General Hospital, Harvard Medical School)

  • Christopher B. Ford

    (Harvard School of Public Health, Boston, Massachusetts 02115, USA)

  • Changwook Min

    (Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School)

  • Rupal R. Shah

    (Harvard School of Public Health, Boston, Massachusetts 02115, USA)

  • Rushdy Ahmad

    (Broad Institute of Harvard and MIT, Cambridge, Massachusetts 02142, USA)

  • Marta Fernandez-Suarez

    (BioMEMS Resource Center, Surgical Services, Massachusetts General Hospital, Harvard Medical School
    Harvard School of Public Health, Boston, Massachusetts 02115, USA
    Present address: Daktari Diagnostics, 85 Bolton Street, Cambridge, Massachusetts 02139, USA)

  • Sarah M. Fortune

    (Harvard School of Public Health, Boston, Massachusetts 02115, USA)

  • Mehmet Toner

    (BioMEMS Resource Center, Surgical Services, Massachusetts General Hospital, Harvard Medical School)

  • Hakho Lee

    (Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School)

  • Ralph Weissleder

    (Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School
    Harvard Medical School)

Abstract

The task of rapidly identifying patients infected with Mycobacterium tuberculosis in resource-constrained environments remains a challenge. A sensitive and robust platform that does not require bacterial isolation or culture is critical in making informed diagnostic and therapeutic decisions. Here we introduce a platform for the detection of nucleic acids based on a magnetic barcoding strategy. PCR-amplified mycobacterial genes are sequence-specifically captured on microspheres, labelled by magnetic nanoprobes and detected by nuclear magnetic resonance. All components are integrated into a single, small fluidic cartridge for streamlined on-chip operation. We use this platform to detect M. tuberculosis and identify drug-resistance strains from mechanically processed sputum samples within 2.5 h. The specificity of the assay is confirmed by detecting a panel of clinically relevant non-M. tuberculosis bacteria, and the clinical utility is demonstrated by the measurements in M. tuberculosis-positive patient specimens. Combined with portable systems, the magnetic barcode assay holds promise to become a sensitive, high-throughput and low-cost platform for point-of-care diagnostics.

Suggested Citation

  • Monty Liong & Anh N. Hoang & Jaehoon Chung & Nil Gural & Christopher B. Ford & Changwook Min & Rupal R. Shah & Rushdy Ahmad & Marta Fernandez-Suarez & Sarah M. Fortune & Mehmet Toner & Hakho Lee & Ral, 2013. "Magnetic barcode assay for genetic detection of pathogens," Nature Communications, Nature, vol. 4(1), pages 1-9, June.
    • Handle: RePEc:nat:natcom:v:4:y:2013:i:1:d:10.1038_ncomms2745
    DOI: 10.1038/ncomms2745
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