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Engineering the type III secretion system in non-replicating bacterial minicells for antigen delivery

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  • Heather A. Carleton

    (Yale University School of Medicine)

  • María Lara-Tejero

    (Yale University School of Medicine)

  • Xiaoyun Liu

    (Yale University School of Medicine)

  • Jorge E. Galán

    (Yale University School of Medicine)

Abstract

Type III protein secretion systems are being considered for vaccine development as virtually any protein antigen can be engineered for delivery by these nanomachines into the class I antigen presentation pathway to stimulate antigen-specific CD8+ T cells. A limitation in the use of this system is that it requires live virulence-attenuated bacteria, which may preclude its use in certain populations such as children and the immunocompromised. Here we report the engineering of the Salmonella Typhimurium type III secretion system in achromosomal, non-replicating nanoparticles derived from bacterial minicells. The engineered system is shown to be functional and capable of delivering heterologous antigens to the class I antigen presentation pathway stimulating immune responses both in vitro and in vivo. This antigen delivery platform offers a novel approach for vaccine development and cellular immunotherapy.

Suggested Citation

  • Heather A. Carleton & María Lara-Tejero & Xiaoyun Liu & Jorge E. Galán, 2013. "Engineering the type III secretion system in non-replicating bacterial minicells for antigen delivery," Nature Communications, Nature, vol. 4(1), pages 1-8, June.
    • Handle: RePEc:nat:natcom:v:4:y:2013:i:1:d:10.1038_ncomms2594
    DOI: 10.1038/ncomms2594
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