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Enhanced dihydropyridine receptor calcium channel activity restores muscle strength in JP45/CASQ1 double knockout mice

Author

Listed:
  • Barbara Mosca

    (General Pathology section, University of Ferrara)

  • Osvaldo Delbono

    (Wake Forest University School of Medicine)

  • Maria Laura Messi

    (Wake Forest University School of Medicine)

  • Leda Bergamelli

    (General Pathology section, University of Ferrara)

  • Zhong-Min Wang

    (Wake Forest University School of Medicine)

  • Mirko Vukcevic

    (Basel University Hospital)

  • Ruben Lopez

    (Basel University Hospital)

  • Susan Treves

    (General Pathology section, University of Ferrara
    Basel University Hospital)

  • Miyuki Nishi

    (Graduate School of Pharmacological Sciences, Kyoto University)

  • Hiroshi Takeshima

    (Graduate School of Pharmacological Sciences, Kyoto University)

  • Cecilia Paolini

    (University Gabriele d Annunzioof Chieti)

  • Marta Martini

    (Physiology and Biophysics University of Ferrara)

  • Giorgio Rispoli

    (Physiology and Biophysics University of Ferrara)

  • Feliciano Protasi

    (University Gabriele d Annunzioof Chieti)

  • Francesco Zorzato

    (General Pathology section, University of Ferrara
    Basel University Hospital)

Abstract

Muscle strength declines with age in part due to a decline of Ca2+ release from sarcoplasmic reticulum calcium stores. Skeletal muscle dihydropyridine receptors (Cav1.1) initiate muscle contraction by activating ryanodine receptors in the sarcoplasmic reticulum. Cav1.1 channel activity is enhanced by a retrograde stimulatory signal delivered by the ryanodine receptor. JP45 is a membrane protein interacting with Cav1.1 and the sarcoplasmic reticulum Ca2+ storage protein calsequestrin (CASQ1). Here we show that JP45 and CASQ1 strengthen skeletal muscle contraction by modulating Cav1.1 channel activity. Using muscle fibres from JP45 and CASQ1 double knockout mice, we demonstrate that Ca2+ transients evoked by tetanic stimulation are the result of massive Ca2+ influx due to enhanced Cav1.1 channel activity, which restores muscle strength in JP45/CASQ1 double knockout mice. We envision that JP45 and CASQ1 may be candidate targets for the development of new therapeutic strategies against decay of skeletal muscle strength caused by a decrease in sarcoplasmic reticulum Ca2+ content.

Suggested Citation

  • Barbara Mosca & Osvaldo Delbono & Maria Laura Messi & Leda Bergamelli & Zhong-Min Wang & Mirko Vukcevic & Ruben Lopez & Susan Treves & Miyuki Nishi & Hiroshi Takeshima & Cecilia Paolini & Marta Martin, 2013. "Enhanced dihydropyridine receptor calcium channel activity restores muscle strength in JP45/CASQ1 double knockout mice," Nature Communications, Nature, vol. 4(1), pages 1-9, June.
    • Handle: RePEc:nat:natcom:v:4:y:2013:i:1:d:10.1038_ncomms2496
    DOI: 10.1038/ncomms2496
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