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A thermoresponsive and chemically defined hydrogel for long-term culture of human embryonic stem cells

Author

Listed:
  • Rong Zhang

    (EaStChem, School of Chemistry, University of Edinburgh
    Present address: School of Materials Science and Engineering, Changzhou University, Changzhou 213164, Jiangsu, China)

  • Heidi K. Mjoseng

    (Scottish Centre for Regenerative Medicine, University of Edinburgh)

  • Marieke A. Hoeve

    (Scottish Centre for Regenerative Medicine, University of Edinburgh)

  • Nina G. Bauer

    (Scottish Centre for Regenerative Medicine, University of Edinburgh)

  • Steve Pells

    (Scottish Centre for Regenerative Medicine, University of Edinburgh)

  • Rut Besseling

    (Merck Sharp & Dohme)

  • Srinivas Velugotla

    (School of Engineering and Electronics, Institute for Integrated Micro and Nano Systems, University of Edinburgh)

  • Guilhem Tourniaire

    (EaStChem, School of Chemistry, University of Edinburgh)

  • Ria E. B. Kishen

    (Scottish Centre for Regenerative Medicine, University of Edinburgh)

  • Yanina Tsenkina

    (Scottish Centre for Regenerative Medicine, University of Edinburgh)

  • Chris Armit

    (Scottish Centre for Regenerative Medicine, University of Edinburgh)

  • Cairnan R. E. Duffy

    (Scottish Centre for Regenerative Medicine, University of Edinburgh)

  • Martina Helfen

    (Institute of Reconstructive Neurobiology, Stem Cell Engineering Group, University of Bonn, Life & Brain Center and Hertie Foundation)

  • Frank Edenhofer

    (Institute of Reconstructive Neurobiology, Stem Cell Engineering Group, University of Bonn, Life & Brain Center and Hertie Foundation)

  • Paul A. de Sousa

    (Scottish Centre for Regenerative Medicine, University of Edinburgh)

  • Mark Bradley

    (EaStChem, School of Chemistry, University of Edinburgh)

Abstract

Cultures of human embryonic stem cell typically rely on protein matrices or feeder cells to support attachment and growth, while mechanical, enzymatic or chemical cell dissociation methods are used for cellular passaging. However, these methods are ill defined, thus introducing variability into the system, and may damage cells. They also exert selective pressures favouring cell aneuploidy and loss of differentiation potential. Here we report the identification of a family of chemically defined thermoresponsive synthetic hydrogels based on 2-(diethylamino)ethyl acrylate, which support long-term human embryonic stem cell growth and pluripotency over a period of 2–6 months. The hydrogels permitted gentle, reagent-free cell passaging by virtue of transient modulation of the ambient temperature from 37 to 15 °C for 30 min. These chemically defined alternatives to currently used, undefined biological substrates represent a flexible and scalable approach for improving the definition, efficacy and safety of human embryonic stem cell culture systems for research, industrial and clinical applications.

Suggested Citation

  • Rong Zhang & Heidi K. Mjoseng & Marieke A. Hoeve & Nina G. Bauer & Steve Pells & Rut Besseling & Srinivas Velugotla & Guilhem Tourniaire & Ria E. B. Kishen & Yanina Tsenkina & Chris Armit & Cairnan R., 2013. "A thermoresponsive and chemically defined hydrogel for long-term culture of human embryonic stem cells," Nature Communications, Nature, vol. 4(1), pages 1-10, June.
    • Handle: RePEc:nat:natcom:v:4:y:2013:i:1:d:10.1038_ncomms2341
    DOI: 10.1038/ncomms2341
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