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Alba shapes the archaeal genome using a delicate balance of bridging and stiffening the DNA

Author

Listed:
  • Niels Laurens

    (VU University, 1081 HV, Amsterdam)

  • Rosalie P.C. Driessen

    (Leiden Institute of Chemistry and Cell Observatory, Leiden University, 2333 CC, Leiden, The Netherlands.)

  • Iddo Heller

    (VU University, 1081 HV, Amsterdam)

  • Daan Vorselen

    (VU University, 1081 HV, Amsterdam)

  • Maarten C. Noom

    (VU University, 1081 HV, Amsterdam)

  • Felix J.H. Hol

    (VU University, 1081 HV, Amsterdam)

  • Malcolm F. White

    (Biomedical Sciences Research Complex, University of St Andrews, St Andrews, Fife KY16 9S, UK.)

  • Remus T. Dame

    (VU University, 1081 HV, Amsterdam
    Leiden Institute of Chemistry and Cell Observatory, Leiden University, 2333 CC, Leiden, The Netherlands.)

  • Gijs J.L. Wuite

    (VU University, 1081 HV, Amsterdam)

Abstract

Architectural proteins have an important role in shaping the genome and act as global regulators of gene expression. How these proteins jointly modulate genome plasticity is largely unknown. In archaea, one of the most abundant proteins, Alba, is considered to have a key role in organizing the genome. Here we characterize the multimodal architectural properties and interplay of the Alba1 and Alba2 proteins using single-molecule imaging and manipulation techniques. We demonstrate that the two paralogues can bridge and rigidify DNA and that the interplay between the two proteins influences the balance between these effects. Our data yield a structural model that explains the multimodal behaviour of Alba proteins and its impact on genome folding.

Suggested Citation

  • Niels Laurens & Rosalie P.C. Driessen & Iddo Heller & Daan Vorselen & Maarten C. Noom & Felix J.H. Hol & Malcolm F. White & Remus T. Dame & Gijs J.L. Wuite, 2012. "Alba shapes the archaeal genome using a delicate balance of bridging and stiffening the DNA," Nature Communications, Nature, vol. 3(1), pages 1-8, January.
    • Handle: RePEc:nat:natcom:v:3:y:2012:i:1:d:10.1038_ncomms2330
    DOI: 10.1038/ncomms2330
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