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Reprogramming to pluripotency is an ancient trait of vertebrate Oct4 and Pou2 proteins

Author

Listed:
  • Natalia Tapia

    (Max Planck Institute for Molecular Biomedicine)

  • Peter Reinhardt

    (Max Planck Institute for Molecular Biomedicine)

  • Annett Duemmler

    (Technische Universität Dresden, DFG Research Center for Regenerative Therapies
    Max Planck Institute of Molecular Cell Biology and Genetics)

  • Guangming Wu

    (Max Planck Institute for Molecular Biomedicine)

  • Marcos J. Araúzo-Bravo

    (Max Planck Institute for Molecular Biomedicine)

  • Daniel Esch

    (Max Planck Institute for Molecular Biomedicine)

  • Boris Greber

    (Max Planck Institute for Molecular Biomedicine)

  • Vlad Cojocaru

    (Max Planck Institute for Molecular Biomedicine)

  • Cynthia Alexander Rascon

    (Technische Universität Dresden, DFG Research Center for Regenerative Therapies
    Max Planck Institute of Molecular Cell Biology and Genetics)

  • Akira Tazaki

    (Technische Universität Dresden, DFG Research Center for Regenerative Therapies
    Max Planck Institute of Molecular Cell Biology and Genetics)

  • Kevin Kump

    (Chandler Medical Center, University of Kentucky)

  • Randal Voss

    (Chandler Medical Center, University of Kentucky)

  • Elly M. Tanaka

    (Technische Universität Dresden, DFG Research Center for Regenerative Therapies
    Max Planck Institute of Molecular Cell Biology and Genetics)

  • Hans R. Schöler

    (Max Planck Institute for Molecular Biomedicine
    University of Münster, Medical Faculty)

Abstract

The evolutionary origins of the gene network underlying cellular pluripotency, a central theme in developmental biology, have yet to be elucidated. In mammals, Oct4 is a factor crucial in the reprogramming of differentiated cells into induced pluripotent stem cells. The Oct4 and Pou2 genes evolved from a POU class V gene ancestor, but it is unknown whether pluripotency induced by Oct4 gene activity is a feature specific to mammals or was already present in ancestral vertebrates. Here we report that different vertebrate Pou2 and Oct4 homologues can induce pluripotency in mouse and human fibroblasts and that the inability of zebrafish Pou2 to establish pluripotency is not representative of all Pou2 genes, as medaka Pou2 and axolotl Pou2 are able to reprogram somatic cells into pluripotent cells. Therefore, our results indicate that induction of pluripotency is not a feature specific to mammals, but existed in the Oct4/Pou2 common ancestral vertebrate.

Suggested Citation

  • Natalia Tapia & Peter Reinhardt & Annett Duemmler & Guangming Wu & Marcos J. Araúzo-Bravo & Daniel Esch & Boris Greber & Vlad Cojocaru & Cynthia Alexander Rascon & Akira Tazaki & Kevin Kump & Randal V, 2012. "Reprogramming to pluripotency is an ancient trait of vertebrate Oct4 and Pou2 proteins," Nature Communications, Nature, vol. 3(1), pages 1-12, January.
    • Handle: RePEc:nat:natcom:v:3:y:2012:i:1:d:10.1038_ncomms2229
    DOI: 10.1038/ncomms2229
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