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A monomeric red fluorescent protein with low cytotoxicity

Author

Listed:
  • I.I. Shemiakina

    (Shemiakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science Miklukho-Maklaya 16/10
    Evrogen JSC, Miklukho-Maklaya 16/10)

  • G.V. Ermakova

    (Shemiakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science Miklukho-Maklaya 16/10)

  • P.J. Cranfill

    (Florida State University, 1800 East Paul Dirac Dr, Tallahassee, Florida 32310, USA)

  • M.A. Baird

    (Florida State University, 1800 East Paul Dirac Dr, Tallahassee, Florida 32310, USA)

  • R.A. Evans

    (Florida State University, 1800 East Paul Dirac Dr, Tallahassee, Florida 32310, USA)

  • E.A. Souslova

    (Shemiakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science Miklukho-Maklaya 16/10)

  • D.B. Staroverov

    (Shemiakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science Miklukho-Maklaya 16/10)

  • A.Y. Gorokhovatsky

    (Shemiakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science Miklukho-Maklaya 16/10)

  • E.V. Putintseva

    (Shemiakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science Miklukho-Maklaya 16/10)

  • T.V. Gorodnicheva

    (Evrogen JSC, Miklukho-Maklaya 16/10)

  • T.V. Chepurnykh

    (Shemiakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science Miklukho-Maklaya 16/10)

  • L. Strukova

    (Shemiakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science Miklukho-Maklaya 16/10)

  • S. Lukyanov

    (Shemiakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science Miklukho-Maklaya 16/10
    Nizhny Novgorod State Medical Academy)

  • A.G. Zaraisky

    (Shemiakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science Miklukho-Maklaya 16/10)

  • M.W. Davidson

    (Florida State University, 1800 East Paul Dirac Dr, Tallahassee, Florida 32310, USA)

  • D.M. Chudakov

    (Shemiakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science Miklukho-Maklaya 16/10
    Nizhny Novgorod State Medical Academy)

  • D. Shcherbo

    (Shemiakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science Miklukho-Maklaya 16/10
    Evrogen JSC, Miklukho-Maklaya 16/10
    Nizhny Novgorod State Medical Academy)

Abstract

Multicolour labelling with fluorescent proteins is frequently used to differentially highlight specific structures in living systems. Labelling with fusion proteins is particularly demanding and is still problematic with the currently available palette of fluorescent proteins that emit in the red range due to unsuitable subcellular localization, protein-induced toxicity and low levels of labelling efficiency. Here we report a new monomeric red fluorescent protein, called FusionRed, which demonstrates both high efficiency in fusions and low toxicity in living cells and tissues.

Suggested Citation

  • I.I. Shemiakina & G.V. Ermakova & P.J. Cranfill & M.A. Baird & R.A. Evans & E.A. Souslova & D.B. Staroverov & A.Y. Gorokhovatsky & E.V. Putintseva & T.V. Gorodnicheva & T.V. Chepurnykh & L. Strukova &, 2012. "A monomeric red fluorescent protein with low cytotoxicity," Nature Communications, Nature, vol. 3(1), pages 1-7, January.
    • Handle: RePEc:nat:natcom:v:3:y:2012:i:1:d:10.1038_ncomms2208
    DOI: 10.1038/ncomms2208
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