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Tyrosine sulfation in a Gram-negative bacterium

Author

Listed:
  • Sang-Wook Han

    (University of California, One Shields Ave, Davis, California 95616, USA
    Chung-Ang University)

  • Sang-Won Lee

    (University of California, One Shields Ave, Davis, California 95616, USA
    Present address: Department of Plant Molecular System Biotechnology & Crop Biotech Institute, Kyung Hee University, Yongin 446-701, Korea)

  • Ofir Bahar

    (University of California, One Shields Ave, Davis, California 95616, USA)

  • Benjamin Schwessinger

    (University of California, One Shields Ave, Davis, California 95616, USA)

  • Michelle R. Robinson

    (1 University Station A5300, University of Texas at Austin)

  • Jared B. Shaw

    (1 University Station A5300, University of Texas at Austin)

  • James A. Madsen

    (1 University Station A5300, University of Texas at Austin)

  • Jennifer S. Brodbelt

    (1 University Station A5300, University of Texas at Austin)

  • Pamela C. Ronald

    (University of California, One Shields Ave, Davis, California 95616, USA
    Kyung Hee University)

Abstract

Tyrosine sulfation, a well-characterized post-translation modification in eukaryotes, has not previously been reported in prokaryotes. Here, we demonstrate that the RaxST protein from the Gram-negative bacterium, Xanthomonas oryzae pv. oryzae, is a tyrosine sulfotransferase. We used a newly developed sulfotransferase assay and ultraviolet photodissociation mass spectrometry to demonstrate that RaxST catalyses sulfation of tyrosine 22 of the Xoo Ax21 (activator of XA21-mediated immunity) protein. These results demonstrate a previously undescribed post-translational modification in a prokaryotic species with implications for studies of host immune responses and bacterial cell–cell communication systems.

Suggested Citation

  • Sang-Wook Han & Sang-Won Lee & Ofir Bahar & Benjamin Schwessinger & Michelle R. Robinson & Jared B. Shaw & James A. Madsen & Jennifer S. Brodbelt & Pamela C. Ronald, 2012. "Tyrosine sulfation in a Gram-negative bacterium," Nature Communications, Nature, vol. 3(1), pages 1-5, January.
    • Handle: RePEc:nat:natcom:v:3:y:2012:i:1:d:10.1038_ncomms2157
    DOI: 10.1038/ncomms2157
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