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Gating of cyclic nucleotide-gated channels is voltage dependent

Author

Listed:
  • Arin Marchesi

    (SISSA (International School for Advanced Studies), Neurobiology Sector, via Bonomea 265, 34136 Trieste, Italy.)

  • Monica Mazzolini

    (SISSA (International School for Advanced Studies), Neurobiology Sector, via Bonomea 265, 34136 Trieste, Italy.
    CBM (Consorzio di Biomedicina Molecolare), Area Science Park, Basovizza, 34149 Trieste, Italy.)

  • Vincent Torre

    (SISSA (International School for Advanced Studies), Neurobiology Sector, via Bonomea 265, 34136 Trieste, Italy.)

Abstract

Cyclic nucleotide-gated channels belong to the family of voltage-gated ion channels, but pore opening requires the presence of intracellular cyclic nucleotides. In the presence of a saturating agonist, cyclic nucleotide-gated channel gating is voltage independent and it is not known why cyclic nucleotide-gated channels are voltage-insensitive despite harbouring the S4-type voltage sensor. Here we report that, in the presence of Li+, Na+ and K+, the gating of wild-type cyclic nucleotide-gated A1 and native cyclic nucleotide-gated channels is voltage independent, whereas their gating is highly voltage-dependent in the presence of Rb+, Cs+ and organic cations. Mutagenesis experiments show that voltage sensing occurs through a voltage sensor composed of charged/polar residues in the pore and of the S4-type voltage sensor. During evolution, cyclic nucleotide-gated channels lose their voltage-sensing ability when Na+ or K+ permeate so that the vertebrate photoreceptor cyclic nucleotide-gated channels are open at negative voltages, a necessary condition for phototransduction.

Suggested Citation

  • Arin Marchesi & Monica Mazzolini & Vincent Torre, 2012. "Gating of cyclic nucleotide-gated channels is voltage dependent," Nature Communications, Nature, vol. 3(1), pages 1-12, January.
    • Handle: RePEc:nat:natcom:v:3:y:2012:i:1:d:10.1038_ncomms1972
    DOI: 10.1038/ncomms1972
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