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The dynamic relationships between the three events that release individual Na+ ions from the Na+/K+-ATPase

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  • David C. Gadsby

    (Marine Biological Laboratory
    Present address: Laboratory of Cardiac/Membrane Physiology, Rockefeller University, 1230 York Avenue, New York, NY 10065, USA (D.C.G.).)

  • Francisco Bezanilla

    (Marine Biological Laboratory
    Present address: Department of Biochemistry and Molecular Biology, University of Chicago, GCIS Building, Room W244, 929 E. 57th Street, Chicago, Illinois 60637, USA (F.B.))

  • Robert F. Rakowski

    (Marine Biological Laboratory)

  • Paul De Weer

    (Marine Biological Laboratory
    Present address: Department of Physiology, University of Pennsylvania, School of Medicine, 3700 Hamilton Walk, Philadelphia, Pennsylvania 19104, USA (P.D.W.))

  • Miguel Holmgren

    (Marine Biological Laboratory
    Present address: Molecular Neurophysiology Section, Porter Neuroscience Research Center, National Institute of Neurological Disorders and Stroke (NINDS), National Institutes of Health (NIH), 35 Convent Dr., Building 35 Room 3B-1016, Bethesda, MD 20892, USA (M.H.))

Abstract

Na+/K+ pumps move net charge through the cell membrane by mediating unequal exchange of intracellular Na+ and extracellular K+. Most charge moves during transitions that release Na+ to the cell exterior. When pumps are constrained to bind and release only Na+, a membrane voltage-step redistributes pumps among conformations with zero, one, two or three bound Na+, thereby transiently generating current. By applying rapid voltage steps to squid giant axons, we previously identified three components in such transient currents, with distinct relaxation speeds: fast (which nearly parallels the voltage-jump time course), medium speed (τm=0.2–0.5 ms) and slow (τs=1–10 ms). Here we show that these three components are tightly correlated, both in their magnitudes and in the time courses of their changes. The correlations reveal the dynamics of the conformational rearrangements that release three Na+ to the exterior (or sequester them into their binding sites) one at a time, in an obligatorily sequential manner.

Suggested Citation

  • David C. Gadsby & Francisco Bezanilla & Robert F. Rakowski & Paul De Weer & Miguel Holmgren, 2012. "The dynamic relationships between the three events that release individual Na+ ions from the Na+/K+-ATPase," Nature Communications, Nature, vol. 3(1), pages 1-6, January.
    • Handle: RePEc:nat:natcom:v:3:y:2012:i:1:d:10.1038_ncomms1673
    DOI: 10.1038/ncomms1673
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