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Propagation stability of self-reconstructing Bessel beams enables contrast-enhanced imaging in thick media

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  • Florian O. Fahrbach

    (Laboratory for Bio- and Nano-Photonics, University of Freiburg, Georges-Koehler-Allee 102, Freiburg 79110, Germany.
    Centre for Biological Signalling Studies (bioss), University of Freiburg, Albertstr19 79104Freiburg, Germany.)

  • Alexander Rohrbach

    (Laboratory for Bio- and Nano-Photonics, University of Freiburg, Georges-Koehler-Allee 102, Freiburg 79110, Germany.
    Centre for Biological Signalling Studies (bioss), University of Freiburg, Albertstr19 79104Freiburg, Germany.)

Abstract

Laser beams that can self-reconstruct their initial beam profile even in the presence of massive phase perturbations are able to propagate deeper into inhomogeneous media. This ability has crucial advantages for light sheet-based microscopy in thick media, such as cell clusters, embryos, skin or brain tissue or plants, as well as scattering synthetic materials. A ring system around the central intensity maximum of a Bessel beam enables its self-reconstruction, but at the same time illuminates out-of-focus regions and deteriorates image contrast. Here we present a detection method that minimizes the negative effect of the ring system. The beam's propagation stability along one straight line enables the use of a confocal line principle, resulting in a significant increase in image contrast. The axial resolution could be improved by nearly 100% relative to the standard light-sheet techniques using scanned Gaussian beams, while demonstrating self-reconstruction also for high propagation depths.

Suggested Citation

  • Florian O. Fahrbach & Alexander Rohrbach, 2012. "Propagation stability of self-reconstructing Bessel beams enables contrast-enhanced imaging in thick media," Nature Communications, Nature, vol. 3(1), pages 1-8, January.
    • Handle: RePEc:nat:natcom:v:3:y:2012:i:1:d:10.1038_ncomms1646
    DOI: 10.1038/ncomms1646
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    Cited by:

    1. Yan Kuai & Junxue Chen & Zetao Fan & Gang Zou & Joseph. R. Lakowicz & Douguo Zhang, 2021. "Planar photonic chips with tailored angular transmission for high-contrast-imaging devices," Nature Communications, Nature, vol. 12(1), pages 1-9, December.

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