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Inhibition of specific gene expressions by protein-mediated mRNA interference

Author

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  • Yoshihiro Yamaguchi

    (Center for Advanced Biotechnology and Medicine, Robert Wood Johnson Medical School)

  • Hirofumi Nariya

    (Center for Advanced Biotechnology and Medicine, Robert Wood Johnson Medical School
    Present address: Department of Microbiology, Faculty of Medicine, Kagawa University, 1750-1 Miki-cho, Kita-gun Kagawa, 761-0793, Japan.)

  • Jung-Ho Park

    (Center for Advanced Biotechnology and Medicine, Robert Wood Johnson Medical School)

  • Masayori Inouye

    (Center for Advanced Biotechnology and Medicine, Robert Wood Johnson Medical School)

Abstract

RNA interference mediated by RNA such as antisense RNA, short interfering RNA and micro RNA is well documented to regulate specific gene expression at the level of messenger RNA. However, RNA interference mediated by proteins has not been reported. Here we identify the MazF-hw mRNA interferase from a superhalophilic archaeon that cleaves RNA at a specific seven-base sequence (UUACUCA). This sequence was found unusually abundant in the mRNAs for rhodopsin transcription activator and some membrane proteins of the archaeon, suggesting that the expression of these proteins is regulated by MazF-hw. When all of the seven-base cleavage sites in essential genes in Escherichia coli were eliminated, the cells were no longer sensitive to MazF-hw, demonstrating that specific gene expression can be regulated by a sequence-specific mRNA interferase. These findings demonstrate that mRNA interference can be mediated not only by RNA but also by proteins to effectively silence specific gene expression in cells.

Suggested Citation

  • Yoshihiro Yamaguchi & Hirofumi Nariya & Jung-Ho Park & Masayori Inouye, 2012. "Inhibition of specific gene expressions by protein-mediated mRNA interference," Nature Communications, Nature, vol. 3(1), pages 1-6, January.
  • Handle: RePEc:nat:natcom:v:3:y:2012:i:1:d:10.1038_ncomms1621
    DOI: 10.1038/ncomms1621
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