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Functionalized arrays of Raman-enhancing nanoparticles for capture and culture-free analysis of bacteria in human blood

Author

Listed:
  • Ting-Yu Liu

    (Institute of Atomic and Molecular Sciences
    Institute of Polymer Science and Engineering, National Taiwan University)

  • Kun-Tong Tsai

    (Institute of Atomic and Molecular Sciences
    Graduate Institute of Photonics and Optoelectronics, National Taiwan University)

  • Huai-Hsien Wang

    (Institute of Atomic and Molecular Sciences
    National Taiwan University)

  • Yu Chen

    (Institute of Atomic and Molecular Sciences)

  • Yu-Hsuan Chen

    (Institute of Atomic and Molecular Sciences)

  • Yuan-Chun Chao

    (Institute of Atomic and Molecular Sciences)

  • Hsuan-Hao Chang

    (Institute of Atomic and Molecular Sciences)

  • Chi-Hung Lin

    (Institute of Microbiology and Immunology, School of Life Science, National Yang-Ming University)

  • Juen-Kai Wang

    (Institute of Atomic and Molecular Sciences
    Center for Condensed Matter Sciences, National Taiwan University)

  • Yuh-Lin Wang

    (Institute of Atomic and Molecular Sciences
    National Taiwan University)

Abstract

Detecting bacteria in clinical samples without using time-consuming culture processes would allow rapid diagnoses. Such a culture-free detection method requires the capture and analysis of bacteria from a body fluid, which are usually of complicated composition. Here we show that coating Ag-nanoparticle arrays with vancomycin (Van) can provide label-free analysis of bacteria via surface-enhanced Raman spectroscopy (SERS), leading to a ~1,000-fold increase in bacteria capture, without introducing significant spectral interference. Bacteria from human blood can be concentrated onto a microscopic Van-coated area while blood cells are excluded. Furthermore, a Van-coated substrate provides distinctly different SERS spectra of Van-susceptible and Van-resistant Enterococcus, indicating its potential use for drug-resistance tests. Our results represent a critical step towards the creation of SERS-based multifunctional biochips for rapid culture- and label-free detection and drug-resistant testing of microorganisms in clinical samples.

Suggested Citation

  • Ting-Yu Liu & Kun-Tong Tsai & Huai-Hsien Wang & Yu Chen & Yu-Hsuan Chen & Yuan-Chun Chao & Hsuan-Hao Chang & Chi-Hung Lin & Juen-Kai Wang & Yuh-Lin Wang, 2011. "Functionalized arrays of Raman-enhancing nanoparticles for capture and culture-free analysis of bacteria in human blood," Nature Communications, Nature, vol. 2(1), pages 1-8, September.
    • Handle: RePEc:nat:natcom:v:2:y:2011:i:1:d:10.1038_ncomms1546
    DOI: 10.1038/ncomms1546
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