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In vitro production of fertile sperm from murine spermatogonial stem cell lines

Author

Listed:
  • Takuya Sato

    (Yokohama City University Graduate School of Medicine)

  • Kumiko Katagiri

    (Yokohama City University Graduate School of Medicine)

  • Tetsuhiro Yokonishi

    (Yokohama City University Graduate School of Medicine)

  • Yoshinobu Kubota

    (Yokohama City University Graduate School of Medicine)

  • Kimiko Inoue

    (RIKEN, Bioresource Center)

  • Narumi Ogonuki

    (RIKEN, Bioresource Center)

  • Shogo Matoba

    (RIKEN, Bioresource Center)

  • Atsuo Ogura

    (RIKEN, Bioresource Center)

  • Takehiko Ogawa

    (Yokohama City University Graduate School of Medicine
    Advanced Medical Research Center, Yokohama City University)

Abstract

Spermatogonial stem cells (SSCs) are the only stem cells in the body that transmit genetic information to the next generation. The long-term propagation of rodent SSCs is now possible in vitro, and their genetic modification is feasible. However, their differentiation into sperm is possible only under in vivo conditions. Here we show a new in vitro system that can induce full spermatogenesis from SSC lines or any isolated SSCs. The method depends on an organ culture system onto which SSCs are transplanted. The settled SSCs form colonies and differentiate up into sperm. The resultant haploid cells are fertile, and give rise to healthy offspring through micro-insemination. In addition, the system can induce spermatogenesis from SSCs that show spermatogenic failure due to a micro-environmental defect in their original testes. Thus, an in vitro system is established that can be used to correct or manipulate the micro-environmental conditions required for proper spermatogenesis from murine SSC lines.

Suggested Citation

  • Takuya Sato & Kumiko Katagiri & Tetsuhiro Yokonishi & Yoshinobu Kubota & Kimiko Inoue & Narumi Ogonuki & Shogo Matoba & Atsuo Ogura & Takehiko Ogawa, 2011. "In vitro production of fertile sperm from murine spermatogonial stem cell lines," Nature Communications, Nature, vol. 2(1), pages 1-7, September.
    • Handle: RePEc:nat:natcom:v:2:y:2011:i:1:d:10.1038_ncomms1478
    DOI: 10.1038/ncomms1478
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    Cited by:

    1. Jiexiang Zhao & Ping Lu & Cong Wan & Yaping Huang & Manman Cui & Xinyan Yang & Yuqiong Hu & Yi Zheng & Ji Dong & Mei Wang & Shu Zhang & Zhaoting Liu & Shuhui Bian & Xiaoman Wang & Rui Wang & Shaofang , 2021. "Cell-fate transition and determination analysis of mouse male germ cells throughout development," Nature Communications, Nature, vol. 12(1), pages 1-20, December.

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